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转录组分析揭示了塞尔托利细胞在山羊的热应激下通过再氧化和代谢途径进行适应

Guang Yang^1,2, Yiwei Wang², Pengyun Ji²

¹College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China.

|January 8, 2025

概括

气候变化影响了牲畜的繁殖. 热应激会损害山羊的塞尔托利细胞 (SCs),改变新陈代谢和应激反应基因. 这项研究确定了开发耐热牲畜的关键基因.

科学领域:

生殖生物学生殖生物学
环境压力生理学生理学
分子内分泌学分子内分泌学

背景情况:

由于气候变化的温度升高会对牲畜的生殖健康产生负面影响,特别是小反动物的丸功能.
羊塞尔托利细胞 (SCs) 对于精子生成至关重要,易受热引起的损伤.

研究的目的:

为了研究山羊塞尔托利细胞 (SCs) 的急性热应激反应.
阐明山羊中因热引起的雄性生殖组织损伤背后的分子机制.

主要方法:

羊的SCs暴露于热应激 (44°C2.5小时).
使用了转录组测序,CCK-8测定,电子显微镜,ROS测量,自检测和西部斑点.
进行了生物信息学分析,包括GO,KEGG和蛋白质与蛋白质相互作用网络.

主要成果:

热应激降低了SC活力,增加了氧化应激和自,改变了基因表达.
确定了1231个差异表达的基因,丰富在膜和代谢途径中.
确定了关键的降低调节代谢基因 (PKLR,ACOT11,LPCT12) 和十个枢纽基因 (HSP90AA1,HSPA5,BAG3,IGF1,HSPH1,IL1A,CCL2,CXCL10,ALB,CALML4).这些基因被发现.

结论:

这项研究揭示了山羊SC对热应激反应的分子机制.
已识别的基因和途径为减轻牲畜繁殖中的热应激提供了目标.
这些发现有助于理解气候变化对畜牧业的影响,并开发耐热牲畜.

关键词:

塞尔托利细胞是什么?自自是自的过程.一只山羊,一只山羊.氧化应激是一种氧化应激.转录组分析转录组分析

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