相关实验视频

Updated: Jun 3, 2025

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

人类桃体衍生的半体干细胞的转录组变化跨文化通道跨文化通道

Moon Sik Oh¹, Heesun Hong¹, Ok Joo Lee¹

¹Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea.

|January 8, 2025

概括

序列亚种植影响桃体衍生的中细胞干细胞 (TMSCs). 第6段被认为是启动分化的一个关键点,受细胞周期基因表达变化的指导.

科学领域:

再生医学是一种再生医学.
干细胞生物学干细胞生物学
分子生物学分子生物学

背景情况:

桃体衍生的介质干细胞 (TMSCs) 由于其增殖和分化能力,在再生医学中表现有前途.
优化TMSC产量和差异化潜力需要了解培养条件.

研究的目的:

调查连续亚种植对TMSC基因表达的影响.
确定最佳通道数量,以保持TMSC差异化能力.

主要方法:

在通道2,4,6和8的TMSCs的基因表达分析.
使用KEGG和Reactome途径进行丰富分析,以确定显著的基因表达变化.

主要成果:

细胞周期基因表达,包括循环素依赖激酶 (CDK) 和循环素,在第6通道达到峰值,并在第8通道下降.
途径分析始终强调细胞循环是所有通道的重要因素.

结论:

细胞周期基因表达动态可以作为TMSC状态的生物标志物.
段落6代表了一个潜在的窗口来诱导TMSCs的分化,以最大限度地发挥其治疗潜力.

关键词:

在体外培养培养过程中,中介细胞干细胞中介细胞干细胞.桃体桃体是一个转录组 (transcriptome) 是一个转录组.

更多相关视频

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Published on: December 24, 2015

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...