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相关概念视频

Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.2K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.2K
Position-effect Variegation02:32

Position-effect Variegation

6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
Light Acquisition02:16

Light Acquisition

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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.4K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K

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相关实验视频

Updated: Jun 3, 2025

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
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Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

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在玉米叶的发育过程中,动态染色素可访问性和基因表达调节.

Yiduo Wang1, Shuai Wang1, Yufeng Wu1

  • 1National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China.

Genes
|January 8, 2025
PubMed
概括
此摘要是机器生成的。

在玉米叶的发育过程中,染色质可访问性动态揭示了基因表达的关键调节作用. 这项研究强调了可访问的染色体区域如何影响跨发育阶段的转录因子结合和基因调节.

关键词:
ATAC-seqq 的使用情况.染色质可访问性 染色质可访问性调节基因表达 调节基因表达玉米叶子的发展转录因子是一种转录因子.

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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In Situ Hybridization for the Precise Localization of Transcripts in Plants
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In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

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相关实验视频

Last Updated: Jun 3, 2025

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
06:11

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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In Situ Hybridization for the Precise Localization of Transcripts in Plants
12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 基因组学就是基因组学.

背景情况:

  • 染色素可访问性对于玉米 (Zea mays) 叶子发育的转录调节至关重要.
  • 染色质可访问性在不同发育阶段的基因表达中的确切作用尚未完全理解.

研究的目的:

  • 调查玉米叶子发育过程中染色质可访问性的动态.
  • 了解染色体可访问性对全基因组基因表达在 BBCH_11,BBCH_13 和 BBCH_17 阶段的影响.

主要方法:

  • 在BBCH_11,BBCH_13和BBCH_17阶段分析了玉米叶.
  • 使用ATAC-seq (使用测序测定转移酶可访问染色体的测试) 评估了染色体可访问性.
  • 用RNA-seq (RNA测序) 进行基因表达的分析,随后进行综合数据分析.

主要成果:

  • 在发育阶段识别了数千个可访问的染色体区域 (ACR),其中一部分靠近转录起点 (TSS).
  • 证明ACR的数量和强度显著影响基因表达水平.
  • 动机分析揭示了涉及叶子发育的转录因子,并确定了具有不同调节模式的基因.

结论:

  • 在玉米叶的发育过程中,染色体的可访问性对空间和时间基因表达调节至关重要.
  • 通过可访问的染色体影响转录因子结合的调节影响基因表达.
  • 这项研究为染色质介导的基因表达和玉米叶的发育提供了新的见解.