在固态发酵过程中对酶和蛋白酶活动以及小麦的物理化学特性进行相关性分析
Hongrui Ren1, Tianli Wang1, Rui Liu1
1State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Foods (Basel, Switzerland)
|January 8, 2025
概括
使用Rhizopus oryzae在55%的水分含量下固态发酵小麦可以增强可溶性食纤维,酶活性和抗氧化特性. 这一过程有效地提升了小麦的价值,改善了其营养和功能特性.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 小麦是一种丰富的农业副产品,其潜力未得到充分利用.
- 固态发酵 (SSF) 为生物处理纤维素基材料提供了一种可持续的方法.
- 麦的提升价值可以改善其营养特征和功能性质.
研究的目的:
- 使用Rhizopus oryzae.优化小麦的固态发酵 (SSF) 条件.
- 评估SSF对可溶性食纤维产量的影响,酶活动 (蛋白酶,氨酶) 和物理化学性质.
- 为了评估小麦发酵后抗氧化化合物和激素清除活动的变化.
主要方法:
- 小麦经过固态发酵,使用Rhizopus oryzae.
- 发酵参数包括含水量 (45-55%) 和时间 (72-144小时) 都有所不同.
- 分析的关键参数包括可溶性食纤维产量,蛋白酶和氨酶活动,总,黄类,基,pH,微观结构和抗氧化能力 (DPPH,ABTS,基清理,减少能力).
主要成果:
- 在96-120小时后,在55%的水分含量下,可溶性食纤维的最佳产量和抗氧化作用得到了实现.
- 蛋白酶和氨基酶活动分别在120小时 (45%水分) 和96小时 (55%水分) 达到峰值.
- 总和黄的显著增加被观察到,在55%的湿度下最佳条件 (在96小时,黄在72小时).
- 微观结构分析显示,在55%的湿度下,在144小时后粉颗粒的降解.
- 基复合素含量显示微小的变化,与pH值变化相关.
结论:
- 用Rhizopus oryzae在55%的水分含量的固体发酵是小麦价值化的有效策略.
- 优化的SSF条件显著增强可溶性食纤维和抗氧化化合物的提取.
- 该过程改善了酶活动,并修改了小麦的物理化学结构,增加了其整体价值.
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