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极端温度对3D打印样本中的人类支气管上皮细胞的影响

Taieba Tuba Rahman¹, Nathan Wood², Zhijian Pei¹

¹Department of Industrial & Systems Engineering, Texas A&M University, College Station, TX 77843, USA.

Bioengineering (Basel, Switzerland)

|January 8, 2025

概括

极端温度会损害人类支气管上皮细胞 (HBE). 增加的热量显著降低了细胞活力,并提高了氧化应激,影响呼吸道组织的健康.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
环境健康科学科学环境健康科学

背景情况:

极端温度可以影响生物系统.
了解细胞对热应激的反应对于呼吸系统健康至关重要.
3D打印为细胞培养和实验提供了新的平台.

研究的目的:

研究极端温度对人类支气管上皮细胞 (HBE) 的影响.
在热挑战下量化细胞活力和氧化应激的变化.
评估3D打印井板对于热应力研究的实用性.

主要方法:

人类支气管上皮 (HBE) 细胞被封装在3D打印的井板中.
样品被暴露在受控温度 (37°C,45°C,55°C) 中10分钟.
细胞活力和氧化应激被评估使用特定的染料 (Hoechst 33342,Sytox,Mitosox) 和板阅读器测量,并补充了显微镜成像.

主要成果:

高温 (45°C和55°C) 显著降低了HBE细胞的活力.
温度升高导致核完整性降低,细胞死亡率提高.
线粒体氧化应激在暴露于45°C和55°C的细胞中升高.

结论:

极端温度对HBE细胞活力产生不利影响,并诱导氧化应激.
3D打印的细胞培养模型为研究呼吸道细胞的热效应提供了一个可行的平台.
这些发现有助于了解呼吸道组织对暴露于热量的反应,并告知潜在的健康风险.

关键词:

通过3D打印打印3D打印.细胞活力细胞的活力.极端温度是指极端的温度.人类支气管上皮细胞线粒体的氧化压力是线粒体的氧化压力.

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