序列抗微生物阶段对根运河微生物组动力学的影响,用于对一次性上性牙周炎进行两次治疗:一项纵向实验研究
Bertan Kesim1, Seda Tezcan Ülger2, Gönül Aslan2
1Department of Endodontics, Faculty of Dentistry, Nuh Naci Yazgan University, Kayseri 38170, Turkey.
Life (Basel, Switzerland)
|January 8, 2025
概括
氧化治疗顶部牙周炎的结果显示,Firmicutes/Bacteroidetes比率降低,Bifidobacterium longum增加,这表明潜在的微生物组变化. 需要进一步的研究来证实这些发现.
科学领域:
- 微生物学 微生物学
- 牙周内科医院 牙周内科医院 牙周内科医院
背景情况:
- 初级顶性牙周炎的有效管理依赖于了解根管微生物组的动态.
- 这项研究检查了两次访问根管治疗中的连续抗菌药物阶段,重点关注氧化药物的影响.
研究的目的:
- 研究连续抗微生物治疗对根运河微生物群的影响.
- 评估氧化药物在调节根管微生物群体中的作用.
主要方法:
- 根管样本分为四个阶段采集:初始感染,后化学机械准备,后内药物治疗和后最后冲洗.
- 使用Illumina MiSeq.进行了16S rRNA基因测序 (V3-V4区域).
- QIIME 2和LEfSe用于数据分析,以识别差异丰富的种类.
主要成果:
- 在治疗阶段之间没有观察到总体微生物群落组成的显著变化.
- 在抗微生物阶段后,Firmicutes/Bacteroidetes的比率下降.
- 特定的细菌,如乳杆菌,甲状细菌,维龙氏菌,Bifidobacterium longum和Dorea formicigenerans在各个阶段显示出差异性的丰富性.
结论:
- 氧化治疗没有显著改变整体根管微生物组成.
- 观察到的趋势,包括减少了Firmicutes/Bacteroidetes比率和增加了Bifidobacterium longum,表明潜在的间接生态变化.
- 需要进行更大规模的研究来验证这些发现,并探索有益细菌在根管治疗中的作用.
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