相关实验视频

Updated: Jun 3, 2025

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Published on: May 13, 2019

弥合差距:内皮功能障碍和iPSC衍生的内皮细胞在疾病建模中的作用

Chiara Sgromo¹, Alessia Cucci¹, Giorgia Venturin¹

¹Department of Health Sciences, School of Medicine, University of Piemonte Orientale, 28100 Novara, Italy.

International journal of molecular sciences

|January 8, 2025

概括

诱导多能干细胞衍生的内皮细胞 (iECs) 为研究内皮功能障碍 (ED) 提供了一个可扩展的模型. 先进的3D和微流体系统提高了iEC模型对疾病研究和药物发现的相关性.

科学领域:

血管生物学血管生物学
干细胞技术干细胞技术
疾病建模疾病建模

背景情况:

内皮细胞 (ECs) 对于血管健康至关重要,但其功能障碍 (ED) 是各种疾病的基础.
初级EC对体外研究有局限性,包括寿命短和供体变异性.
内皮功能障碍涉及氧化应激,炎症和改变的血管透性.

研究的目的:

审查从诱导多能干细胞 (iPSCs) 衍生的诱导内皮细胞 (iECs) 的应用,作为内皮功能障碍 (ED) 的模型.
突出3D和微流体系统的进步,以创建更具生理相关性的iEC模型.
讨论iEC模型在研究各种血管和非血管疾病中ED的潜力.

主要方法:

使用诱导多能干细胞 (iPSC) 来产生诱导内皮细胞 (iEC).
采用3D培养系统和微流体设备来模拟血管微环境.
审查关于iEC应用在模拟内皮功能障碍中的当前文献.

主要成果:

iECs为ED研究提供无限,一致的内皮细胞来源.
3D和微流体模型增强了基于iEC的ED研究的生理相关性.
iEC模型适用于研究糖尿病,心血管和神经退行性疾病等疾病中的ED.

结论:

诱导内皮细胞 (iECs) 代表了对内皮功能障碍 (ED) 建模的重大进步.
增强的3D和微流体系统提高了iEC模型在疾病研究中的实用性.
iEC技术有望促进我们对ED相关疾病的理解和治疗.

关键词:

心血管疾病心血管疾病糖尿病糖尿病患者糖尿病患者它们是内皮细胞.内皮质功能障碍在内皮质功能障碍诱导多能干细胞的诱导干细胞.神经退行性疾病的神经退行性疾病

更多相关视频

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Published on: November 6, 2019

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

相关概念视频

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...