相关实验视频

Updated: Jun 3, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

在Streptomyces clavuligerus中识别小RNA,使用高分辨率的转录组学和在克拉夫兰酸生产过程中的表达概况

Carlos Caicedo-Montoya¹, Luisa F Patiño¹, Rigoberto Ríos-Estepa²

¹Grupo de Bioprocesos, Departamento de Ingeniería Química, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín 050010, Colombia.

International journal of molecular sciences

|January 8, 2025

概括

研究人员在Streptomyces clavuligerus中发现了新的小型非编码RNA (sRNA),这些RNA调节了代谢过程. 两个特定的sRNA向了克拉武兰酸生物合成基因,为代谢工程提供了提高产量的潜力.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

小型非编码RNAs (sRNAs) 是各种生物体中代谢途径的关键调节者.
关于Streptomyces clavuligerus中sRNA的信息有限,Streptomyces clavuligerus是一个关键的抗生素生产者.

研究的目的:

在Streptomyces clavuligerus中使用生物信息学和RNA-seq数据识别和描述新的小型非编码RNA (sRNA).
调查这些sRNAs在调节酸 (CA) 生产中的作用.

主要方法:

对具有稳定的二次结构的保护性基因间区域 (IGRs) 的生物信息分析.
转录组组装和分析来自S. clavuligerus. 的RNA-seq数据.
在克劳兰酸生产过程中对假定sRNA的差异表达分析.
相互作用组分析以确定差异表达sRNA的mRNA标.

主要成果:

确定了606种保存的IGR,预计有272种含有非编码RNA.
在分析条件下,确认了基因组功能与完整的基因表达.
发现差异表达的sRNA作为全球调节器,具有多个mRNA标.
发现了两种特定的sRNA向克拉夫兰酸 (CA) 生物合成基因集群.

结论:

在S. clavuligerus中的新型sRNA作为全球调节剂,影响二次代谢产生的功能.
通过准其基因集群,两个已识别的sRNA直接调节CA生物合成.
这些sRNA代表了代谢工程的有希望的目标,以提高克拉夫兰酸的产量.

关键词:

这种类型是Streptomyces clavuligerus.生物合成基因集群生物合成基因集群克拉夫兰酸是什么克拉夫兰酸是什么小小的RNARNA小小的RNARNA.翻译学翻译学翻译学翻译学

更多相关视频

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...