对宿主内部进化和口腔和肠道链球菌的动态的洞察揭示了利基适应
Mohamed M H Abdelbary1,2, Maximilian Hatting3, Andrea Dahlhausen4
1Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode Branch, 38855 Wernigerode, Germany.
International journal of molecular sciences
|January 8, 2025
概括
链球菌通过遗传变化和移动元素适应口腔和肠道环境. 主体内部的进化显示出不同的口腔和肠道血统,受个人因素的影响,并始于口腔.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 主体-微生物相互作用
背景情况:
- 口腔-肠轴连接口腔和胃肠道,影响宿主健康和微生物组成.
- 链球菌在同一宿主中殖民口腔和肠道的特点是独特的.
- 了解链球菌的适应对于破译口腔-肠轴动态至关重要.
研究的目的:
- 调查链球菌在个体内殖民口腔和肠道的遗传变化和适应机制.
- 来自炎症性肠病 (IBD) 患者和健康对照的口腔和便样本中的链球菌种群进行比较.
- 阐明单一宿主内的链球菌的微演变路径和遗传分歧.
主要方法:
- 从唾液和便样本中分离的218种链球菌的全基因组测序 (WGS).
- 链球菌物种的识别和流行率分析.
- 比较基因组学分析遗传适应,移动遗传元素 (MGE) 和特定基因 (例如,tetA).
- 核心基因组单核酸多态性 (SNP) 分析以评估遗传差异.
主要成果:
- 确定了16种链球菌种,其中*Streptococcus infantis*在口腔中占主导地位,并在IBD患者的肠道中普遍存在.
- 在这两组中, *S. parasanguinis* 的肠道患病率高于口服患病率.
- 通过重组和MGE获取,通过宿主内部的微演变,肠道分离物具有更完整的菌体和*tetA*基因.
- 核心基因组SNP分析显示,个体内口腔和肠道链球菌之间存在显著的遗传差异.
- 在肠道基因组中确定了利基特定的突变特征,这表明了不同的克隆血统和个体依赖的进化.
结论:
- 链球菌在宿主中表现出显著的微观进化和口腔和肠道环境之间的利基专业化.
- 适应包括重组,MGE获取 (菌体, *tetA*) 和不同的突变特征.
- 宿主内链球菌的进化是个体依赖的,起源于口腔,并适应肠道位.
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