大麻比对人体天氨酸毒性的保护作用 - - 实验室研究
Eryka Pankowska1, Oliwia Kończak1, Paula Żakowicz1
1Students Scientific Society of Veterinary Medicine, Section of Veterinary Pharmacology and Toxicology "Paracelsus", Faculty of Veterinary Medicine and Animal Sciences, Poznan University of Life Sciences, 60-637 Poznan, Poland.
International journal of molecular sciences
|January 8, 2025
概括
Cannabidiol (CBD) 可能会减轻兽医药物 tiamulin 对人类细胞的有害影响. 研究表明,CBD可以降低 tiamulin.
科学领域:
- 食品毒理学 食品毒理学
- 兽医药理学 兽医药理学 兽医药理学
- 细胞生物学 细胞生物学
背景情况:
- 食品中的兽药残留物,如 tiamulin,对公众健康构成风险.
- 来自Cannabis sativa L. 的大麻二醇 (CBD) 正在探索减轻药物的不良影响.
- 了解 tiamulin 对人类细胞的影响对于食品安全至关重要.
研究的目的:
- 为了研究CBD对人类细胞系中 tiamulin诱导的细胞毒性的作用.
- 评估CBD对 tiamulin细胞不良影响的保护潜力.
- 为了确定 tiamulin 和 CBD 之间的相互作用动态.
主要方法:
- 暴露SH-SY5Y,HepG2和HEK-293细胞系72小时的 tiamulin 和 tiamulin/CBD混合物.
- 使用终点进行细胞毒性评估:线粒体/溶酶体活性,增殖,膜完整性,DNA合成.
- 评估氧化应激,细胞亡,细胞形态和组合指数,用于相互作用分析.
主要成果:
- 单独的tiamulin抑制了特定细胞系中的溶酶体,线粒体活性和DNA合成.
- 泰米林/CBD混合物显示出更高的IC50值,表明细胞毒性降低.
- 与单独使用 tiamulin 相比,与CBD联合使用减少了氧化应激,细胞亡和细胞形态的改变.
结论:
- CBD在人体细胞中显示出对 tiamulin 的细胞毒性有保护作用.
- tiamulin 和 CBD 之间的相互作用依赖于度,从对抗性到协同性.
- 这些发现强调了CBD在食品毒理学和减轻兽药残留物风险方面的潜在作用.
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