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Toxicity Testing in Animals01:23

Toxicity Testing in Animals

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Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
222

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胚胎体检:一种简单可靠的替代性发育毒性测试

Inho Hwang1, Eui-Bae Jeung1

  • 1Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea.

International journal of molecular sciences
|January 8, 2025
PubMed
概括
此摘要是机器生成的。

胚胎体检测 (EBT) 提供了一种更快,更简单,更准确的替代动物试验,用于发育性毒性查. 这种经过验证的体外试验方法取代了复杂的测试,满足了对伦理和高效毒理学评估的日益增长的需求.

关键词:
替代性测试试验 替代性测试试验发展性毒性 发展性毒性胚胎体检 (EBT) 是一种胚胎体检.

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科学领域:

  • 毒理学 毒理学 毒理学
  • 发展生物学 发展生物学
  • 在体外检测中进行检测.

背景情况:

  • 越来越重视动物福利和伦理考虑,推动了对替代测试方法的需求.
  • 传统的发展性毒性动物试验是耗时的,昂贵的和复杂的.
  • 现有的体外试验方法,如胚胎干细胞试验 (EST),在持续时间和复杂性方面存在局限性.

研究的目的:

  • 引入和验证胚胎体检测 (EBT) 作为 EST 的更有效的替代方案,用于发育毒性查.
  • 提供一种更简单,更准确,更可复制的体外方法来评估早期发育性毒性.

主要方法:

  • 胚胎体检测 (EBT) 使用小鼠胚胎干细胞.
  • 毒性是通过测量胚胎身体区域的变化来评估的,不像EST的重点是心脏分化和心跳.
  • 该EBT程序的设计是直接的,并有一个更短的测试期.

主要成果:

  • EBT表现出高准确性和可重复性.
  • 该测试经过了两轮验证,证实了其可靠性.
  • 在毒理学查中,EBT已经准备好用于实际应用.

结论:

  • 胚胎体检测 (EBT) 是一种经过验证的,实用的替代动物发展毒性测试方法.
  • 它的简单性,速度和准确性使它适合用于查早期发育性毒性.
  • 该EBT已经准备好满足对毒理学中非动物试验方法的日益增长的需求.