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Updated: Jun 14, 2026

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在分子拥挤条件下使用ANS染料的陷

Sergey A Silonov1, Alexander I Kuklin2, Semen V Nesterov1

  • 1Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 St. Petersburg, Russia.

International journal of molecular sciences
|January 8, 2025
PubMed
概括

拥挤剂和瓜尼丁化 (GdnHCl) 影响1-anilino-8-naphthalenesulfonate (ANS) 染料,使蛋白质折叠研究复杂化. 这项研究阐明了这些相互作用,并突出了在拥挤,变性条件下ANS的局限性.

关键词:
一年多的时间.在 GdnHClCl 中.拥挤代理人的人群.光疏水性染料是一种光染料.大分子分子拥挤.蛋白质的结构转换.

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Aryldiazonium Salts to Azo Dyes: Diazo Coupling

The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.