进化保存信号分子的微调表达在Ciona的Notochord中
Lenny J Negrón-Piñeiro1, Yushi Wu1, Ravij Mehta2
1Department of Molecular Pathobiology, New York University College of Dentistry, 345 E 24th Street, New York, NY 10010, USA.
International journal of molecular sciences
|January 8, 2025
概括
节弦管对于节弦管发育至关重要,在组织模式中演变出了新的角色. 研究人员研究了西奥纳带的信号分子,以了解这些进化变化.
科学领域:
- 发育生物学 发展生物学
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 笔弦是弦状动物发育中的重要轴状结构,从衣动物到人类至关重要.
- 除了其结构性作用之外,在整个弦状细胞的进化过程中,记号带已经获得了重要的组织模式功能.
- 这种进化涉及到信号通路的招募和新的分子机制,用于精确的时间和空间控制.
研究的目的:
- 为了研究诺托琴带模式角色的进化轨迹.
- 为了调查Ciona. tunicate的 notochord 内部的信号分子表达.
- 为了识别控制基因表达的cis-regulatory元素.
主要方法:
- 在Ciona笔记带中信号分子的表达分析.
- 标识和表征符号增强剂.
- 与保存的信号基因相关的 cis 调节元件的解构.
主要成果:
- 几种编码信号通路组件的基因在Ciona的诺托霍德发育过程中得到表达.
- 一些信号基因在记忆带内表现出区域化和/或谱系特异的表达模式.
- 鉴定了TGF-β和Ctgf的新型记弦增强剂,揭示了它们表达模式的cis-regulatory起源.
结论:
- 这项研究揭示了背后的分子机制的演变的 notochord 模式的功能.
- 在Ciona的发现为保存的状动物的发育过程提供了洞察力.
- 已识别的增强剂为理解在心弦发展中的信号调节提供了基础.
相关概念视频
Notch Signaling Pathway
4.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K
Non-Canonical Wnt Signaling Pathways
7.2K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Determination
18.2K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
18.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K


