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SPHK1的免疫调节作用及其与黄色鼓 (Nibea albiflora) 中的TFAP2A的相互作用

Yu Cui¹, Shuai Luo¹, Baolan Wu¹

¹State Key Laboratory of Mariculture Breeding, Key Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Xiamen 361021, China.

International journal of molecular sciences

|January 8, 2025

概括

研究人员在黄色鼓鱼中确定了Ydsphk1基因,这对免疫反应和对Vibrio harveyi的抵抗至关重要. 这一发现为改善水产养殖疾病管理策略提供了潜力.

科学领域:

水产养殖免疫学水产养殖免疫学
鱼类疾病耐药性鱼类疾病耐药性
免疫调节的分子机制

背景情况:

斯芬戈辛激酶 (SPHKs) 产生斯芬戈辛-1-酸盐 (S1P),对于炎症和免疫反应至关重要.
黄鼓 (Nibea albiflora) 对Vibrio harveyi的敏感性需要了解遗传抵抗因素.

研究的目的:

为了识别与黄鼓对Vibrio harveyi.yi.抗性相关的基因.
阐明Ydsphk1基因在免疫调节中的功能及其分子相互作用.

主要方法:

全基因组关联分析 (GWAS) 以确定候选基因.
Ydsphk1基因克隆,表达和亚细胞局部化研究.
RNA测序 (RNA-Seq) 用于在Ydsphk1过度表达时识别差异表达的基因.
促进体分析和转录因子结合位点识别 (TFAP2A).

主要成果:

GWAS发现Ydsphk1与黄鼓对Vibrio harveyi的抵抗有显著的关联.
Ydsphk1编码在细胞质中局部化的蛋白质,含有DAGKc域.
过度表达Ydsphk1改变了关键免疫相关基因的表达,包括对nsun5和hsp90aa1的上调和对Nfkbia和hmox1.1的下调.
在Ydsphk1促进体中确定了TFAP2A结合部位,TFAP2A对其活动进行了负面调节.

结论:

Ydsphk1在调节黄色鼓中的免疫反应中发挥着关键作用,影响NF-κB信号传递和铁亡等途径.
Ydsphk1基因是增强水产养殖中病原体耐药性的潜在目标.
这项研究为开发水产养殖物种新型疾病管理策略提供了基础的见解.

关键词:

尼贝亚白花科 (Nibea albiflora) 是一种植物.有关RNA测序的RNA测序这种病毒是Vibrio harveyi.免疫调节免疫调节斯芬戈氨酸激酶的作用在SPHK1上

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