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在羊群中,LPS通过抑制STAT1酸化来破坏子宫内膜受体性

Xing Fan¹, Jinzi Wei¹, Yu Guo¹

¹College of Animal Science and Technology, Northeast Agricultural University, Harbin 150038, China.

International journal of molecular sciences

|January 8, 2025

概括

来自格拉姆阴性细菌的脂聚糖 (LPS) 通过JAK2/STAT1通路破坏子宫内膜受容性,从而损害绵羊胚胎的植入. 这导致受孕率降低和怀孕失败.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
遗传学是一个遗传学.

背景情况:

子宫感染会对反动物的繁殖效率产生负面影响.
格拉姆阴性细菌感染,特别是脂聚糖 (LPS) 暴露,导致生殖功能障碍,包括植入失败和受孕率降低.

研究的目的:

调查LPS输液在关键胚胎植入期期间对羊的子宫内膜接受度的影响.
阐明分子机制,特别是JAK2 / STAT1通路,涉及LPS诱导的子宫内膜受体性损伤.

主要方法:

在12日,16日和20日对孕羊进行体内LPS输注.
用激素和LPS进行羊子宫内膜上皮细胞的体外治疗.
与植入,前列腺素和JAK/STAT通路相关的基因和蛋白质表达的分析.
使用p-STAT1抑制剂 (fludarabine) 的验证.

主要成果:

输入LPS改变了前列腺素,粘附因子,关键植入基因 (HOXA10,HOXA11,LIF) 和延长基因 (ISG15,RSAD2,CXCL10) 的表达.
LPS显著降低了p-STAT1和增加了IRF9蛋白水平,表明了JAK2/STAT1通路的参与.
实验室模型证实,LPS和p-STAT1抑制降低了前列腺素合成,并抑制了关键植入基因表达.

结论:

妊娠12日,16日和20日的LPS输液会损害绵羊的子宫内膜受体.
JAK2/STAT1通路是羊中LPS诱导的生殖衰竭的关键调解者.
准JAK2/STAT1通路可能为LPS相关的怀孕流产提供治疗策略.

关键词:

在LPS中使用LPS.美国国家统计局 (STAT1)胚胎植入胚胎的植入宫内膜的受体性宫内膜的受体性羊群羊群羊群的羊群是什么意思

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