通过聚合酶链反应选择GABA生产的乳酸细菌菌株,使用新B和C多种原料开发新的功能性食品
Susana Langa1, Silvia Santos1, José Antonio Flores1
1Food Technology Department, National Institute for Agricultural and Food Research and Technology (INIA-CSIC), Carretera de La Coruña Km 7.5, 28040 Madrid, Spain.
International journal of molecular sciences
|January 8, 2025
概括
一种新的PCR方法有效地检测乳酸细菌 (LAB) 中的gadB基因,与胺酸 (GABA) 生产有很强的相关性. 这有助于选择LAB用于开发富含GABA的功能性食品.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 食品科学 食品科学 食品科学
背景情况:
- 胺黄油酸 (GABA) 是一种具有健康益处的生物活性化合物,使其成为功能性食品的有价值成分.
- 乳酸细菌 (LAB) 可以通过谷氨酸脱酶 (GadB) 和谷氨酸/谷氨酸抗体 (GadC) 生产GABA.
研究的目的:
- 开发一种使用聚合酶链反应 (PCR) 的分子查方法,以检测LAB物种中的gadB和gadC基因.
- 建立一个可靠的策略来识别能够高GABA生产的LAB菌株,用于功能性食品应用.
主要方法:
- 设计了新型多种原料,用于基于PCR检测六种LAB菌株的92个LAB菌株中的B基因和C基因.
- 评估了谷氨酸脱碳酶活性 (GAD测定) 和GABase酶量的量化.
- 与酶分析和GABA生产水平相关的PCR结果.
主要成果:
- 在多个LAB物种中发现了gadB基因,包括Lactiplantibacillus plantarum和Lactococcus lactis.
- 93.2%具有谷氨酸脱碳酶活性的菌株携带了gadB基因.
- 对gadB的PCR检测与GABA生产有很强的相关性,在阳性菌株中观察到高产量.
结论:
- 对gadB基因的PCR检测是选择具有高GABA产生潜力的LAB菌株的有效方法.
- 这种分子选方法促进了用GABA强化的功能性食品的开发.
相关概念视频
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...


