可转移元素有助于Drosophila melanogaster中的长非编码RNA的调节
Yuli Gan1,2, Lingyan Wang1, Guoxian Liu2
1College of Life Science, Hebei University, Baoding 071002, China.
Insects
|January 8, 2025
概括
可转移元素 (TE) 显著促进了Drosophila中的长非编码RNA (lncRNA) 调节. 这些元素影响lncRNA转录,表观遗传修饰和表达模式,揭示了新的调节机制.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 可转移元素 (TE) 和非编码序列是基因组的关键组成部分,但它们在长非编码RNA (lncRNAs) 中的作用尚不清楚.
- 虽然TE-lncRNAs在物种中发现,但它们的特征和调节功能,特别是在昆虫中,仍然在很大程度上未被探索.
- 这项研究研究了Drosophila melanogaster中的TE-lncRNAs,重点关注跨多个omics级别的转位子影响.
研究的目的:
- 使用集成的多omics数据来描述Drosophila melanogaster中的TE-lncRNAs.
- 探索转体子对 lncRNA 调节在转录,后转录和表观遗传层面的功能性贡献.
- 了解TE插入在压力条件下对lncRNA表达,保存和调节网络的影响.
主要方法:
- 整合了256个公共RNA-seq样本和15个来自Drosophila S2细胞的lncRNA-seq样本.
- 识别与lncRNA序列重叠的转子.
- 分析多omics数据,包括染色质可访问性,转录因子结合,m6A甲基化和小型开放的读取框架.
主要成果:
- 确定了219个TE-lncRNAs (占所有lncRNA的40.4%),其中67.2%含有多个TE;LTR/Gypsy是最常见的插入.
- 转位子优先插入促进体,转录起点和内基区域,特别是在染色体末端.
- 与其他lncRNA相比,TE-lncRNA的长度更长,保存率更低,表达特异性更高.
- 在转子插入和染色质开放之间发现了正相关性;516个TE-lncRNA提供了转录因子结合点,在重金属应力下重新连接调节网络.
- 99个TE-lncRNA与m6A甲基化位点相关,115个可能提供了小型开放的读取框架.
结论:
- 可转移元素对长非编码RNAs的调节有显著的贡献.
- 转录酶促进了 lncRNAs 的转录调节.
- 此外,TE还促进lncRNAs的转录后和表观遗传调节,影响基因表达和细胞对压力的反应.
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