生物测试引导的隔离和识别来自入侵的天堂树树干和树干树皮的抗菌化合物
Anna Cselőtey1, Márton Baglyas1,2, Nóra Király1
1Plant Protection Institute, HUN-REN Centre for Agricultural Research, Fehérvári út 132-144, 1116 Budapest, Hungary.
Molecules (Basel, Switzerland)
|January 8, 2025
概括
研究人员从天堂树树皮中分离出6种化合物,其中5种是植物新发现的. 大多数化合物表现出对细菌细菌的抗菌活性,其中丁-6-one是最有效的.
科学领域:
- 自然产品化学 自然产品化学
- 药理学是指药理学,即药理学是指药理学.
- 微生物学 微生物学
背景情况:
- 天堂树 (Ailanthus altissima) 是一种具有传统药用历史的植物.
- 从A.altissima中识别生物活性化合物可以导致新的治疗剂.
- 了解植物化合物的抗菌性质对于开发新型抗菌药物至关重要.
研究的目的:
- 从Ailanthus altissima茎和树干树皮提取物中分离和识别化学成分.
- 评估分离化合物的抗菌活性,以对抗 Bacillus subtilis 的作用.
- 报告关于在A. altissima.中存在特定化合物及其生物活性的新发现.
主要方法:
- 闪光列色谱被用于植物提取物的分化.
- 用薄层染色学 (TLC) 引导的生物测试 (TLC-Bacillus subtilis测试) 和质谱学 (TLC加热电喷射高分辨率联质谱 - HESI-HRMS/MS) 用于化合物识别.
- 使用HESI-HRMS/MS和核磁共振 (NMR) 光谱进行了结构阐明. 通过微稀释试验评估了抗菌活性.
主要成果:
- 分离了六种已知的化合物: (9Z,11E) -13-基 -9,11-octadecadienoic 酸 (13-HODE,A1), (10E,12Z) -9-基 -10,12-octadecadienoic 酸 (9-HODE,A2),六氧化甘酸 (thapsic 酸,A3),16-hydroxyhexadecanoic 酸 (木酸,A4),16-feruloyloxypalmitic 酸 (alpinagalanate,A5),和丁-6-one (A6).
- 在A. altissima中首次发现了A1-A5化合物.
- 除A5外,所有分离的化合物都表现出对B. subtilis的抗菌活性. 康-6-一 (A6) 具有最强的活性,最小抑制度 (MIC) 为8.3μg/mL. 最近报告了A3和A4的抗菌活性.
结论:
- 该研究成功地从A. altissima树皮中分离和识别了六种化合物,扩大了该植物已知的植物化学特征.
- 这些发现突出了A. altissima成分作为抗菌剂来源的潜力,特别是丁-6-one,酸和酸.
- 这项研究为从天堂树中提取的化合物的化学多样性和生物活动提供了新的知识.
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