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在Enterococcus faecalis中增强消化酶活性使用ARTP变异酶

Meng Yuan^1,2, Zhengzhong Li², Qunlan Zhou^2,3

¹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.

|January 8, 2025

概括

大气和室温等离子体 (ARTP) 产生了三种新的Enterococcus faecalis益生菌菌株. 这些增强的菌株表现出优越的消化酶活性和稳定性,提供了宝贵的微生物资源.

科学领域:

微生物学微生物学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

Enterococcus faecalis是食品补充剂中公认的益生菌之一.
大气和室温等离子体 (ARTP) 是微生物突变查的有效工具.

研究的目的:

使用ARTP.获得高产的消化酶突变菌株的E. faecalis.
描述所获得的突变菌株的酶,稳定性和安全性.

主要方法:

E. faecalis 接受了 ARTP 治疗.
突变菌株被选为增强的α-氨酶,脂酶和中性蛋白酶活性.
评估了酶活性,稳定性,自我聚合,疏水性,模拟肠液中的生存,抗氧化能力和Macrobrachium rosenbergii的安全性.

主要成果:

产生了833个突变菌株;三个菌株 (EF-448,EF-798,EF-804) 显示了显著增加的酶活性 (180%的α-氨基酶,30%的脂酶,>40%的蛋白酶).
在九代人中,酶活性保持稳定.
突变菌株表现出高自聚性 (>91%),疏水性 (>93%),在模拟肠液中存活 (约. 85%),保留了抗氧化能力,并且没有表现出血解.
在Macrobrachium rosenbergii中进行的安全试验表明没有死亡率.

结论:

ARTP突变发生成功地产生了三种E. faecalis突变菌株,具有增强和稳定的消化酶生产.
这些突变菌株具有理想的益生菌特性,包括高生存率和抗氧化能力.
这项研究为益生菌应用提供了有效的微生物资源.

关键词:

抗氧化能力的抗氧化能力.大气和室温等离子体.肠球菌 (enterococcus faecalis) 是一个可怕的病菌.脂酶脂酶的使用方法蛋白质酶蛋白质酶是一种蛋白质.α-氨基酸氨基酶的使用.

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