解读与ALV-J和CIAV同时感染的的免疫调节:一种转录基因方法
Sheng Chen1,2,3, Huijuan Xu1, Wenxue Li1
1State Key Laboratory of Swine and Poultry Breeding Industry & Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Microorganisms
|January 8, 2025
概括
禽类白血病病毒亚组J (ALV-J) 和贫血病毒 (CIAV) 联合感染通过氧化应激和明显的免疫逃避抑制家禽免疫力. 了解这些分子通路有助于制定有针对性的家禽疾病控制策略.
科学领域:
- 禽类病毒学 禽类病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 病毒共感染,特别是禽类白血病病毒亚组J (ALV-J) 和感染性贫血病毒 (CIAV),在家禽行业造成重大经济损失.
- 这些共同感染会损害的免疫防御,增加对二次感染的易感性,并降低疫苗的疗效.
- 以前的研究已经探索了病原性和免疫抑制,但关键的分子机制仍然不清楚.
研究的目的:
- 在中研究ALV-J和CIAV联合感染的协同分子效应.
- 为了确定关键的基因和分子途径参与免疫抑制引起的这些病毒共感染.
- 阐明在共感染期间采用的不同免疫逃避策略.
主要方法:
- 使用高通量测序进行全面的转录组分析.
- 差异表达mRNAs (DEmRNAs) 和miRNAs (DEmiRNAs) 的识别和分析.
- 研究与氧化应激和rRNA代谢相关的途径.
主要成果:
- 确定了1007个与协同激活相关的DEmRNA和62个DEmiRNA,以及与特定激活过程相关的331个DEmRNA和62个DEmiRNA.
- 观察到反应性氧物种 (ROS) 和氧化应激通路的利用增加,有助于免疫抑制.
- 与单个感染相比,检测到不同的免疫逃避策略,涉及与共感染的rRNA代谢调节.
结论:
- 与ALV-J和CIAV的同时感染协同影响宿主基因和miRNA的表达,导致免疫抑制.
- 氧化应激和改变的rRNA代谢是这些病毒共感染的免疫抑制作用的关键分子机制.
- 这些发现为开发向治疗和改善家禽疾病控制策略提供了关键的见解,以减轻经济损失.
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