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从Saccharomyces cerevisiae生物质中通过热和非热技术释放生物聚合物
Marianna Ciccone1, Muhammad Rehan Khan1, Junior Bernardo Molina Hernandez1
1Department of Agricultural and Food Sciences, Campus of Food Science, Alma Mater Studiorum, University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy.
Microorganisms
|January 8, 2025
概括
高压同质化 (HPH) 和脉冲电场 (PEF) 显著改善了酵母细胞壁的提取,例如β-葡萄糖. 这些方法为食品包装应用的生物聚合物生产提供了可持续的途径.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 材料科学 材料科学 材料科学
背景情况:
- 酵母细胞壁的成分,特别是β-葡萄糖和曼诺蛋白,对于制造可持续的生物聚合物非常有价值.
- 酵母细胞壁的复杂结构对有效提取这些有价值的成分提出了挑战.
研究的目的:
- 研究高压同质化 (HPH) 和脉冲电场 (PEF) 的有效性,有或没有热处理 (TT),用于从面包厂酵母 (BY) 和酒厂废酵母 (BSY) 中提取组件.
- 评估这些治疗对细胞壁破坏和随后的碳水化合物,蛋白质,β-葡萄糖和曼诺蛋白释放的影响.
主要方法:
- 高压同质化 (HPH) 和脉冲电场 (PEF) 单独或与热处理 (TT) 结合使用.
- 微观评估酵母细胞壁的破坏.
- 在经过处理的酵母生物质中量化碳水化合物,蛋白质,β-葡萄糖和曼诺蛋白.
主要成果:
- HPH导致了细胞的完整分解,并增强了细胞内成分的释放.
- 产品环境污染物主要诱导细胞膜透.
- 结合HPH和PEF治疗导致了显著的细胞壁应力和部分分解,显著改善了β-葡萄糖的回收 (BSY中最高可达10.44g/100g干物质).
结论:
- HPH和PEF是破坏酵母细胞壁的有效技术,并增强像β-葡萄糖这样的有价值成分的恢复.
- 这些方法为从酵母生物质中生产用于食品包装的生物聚合物提供了可行和可持续的方法.
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