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对大肠杆菌 (Escherichia coli) 的分子分析以及来自不同来源的族群和序列类型之间的相关性

João Gabriel Material Soncini¹, Vanessa Lumi Koga¹, Bruna Fuga²

¹Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, State University of Londrina, Londrina 86057-970, Paraná, Brazil.

|January 8, 2025

概括

确定了抗生素耐药的大肠杆菌克隆,包括人类的ST131和肉类中的ST38. 关键的耐药性基因和毒性因素普遍存在,突出显示了食物传播的风险.

科学领域:

微生物学微生物学
遗传学遗传学是一个
公共卫生公共卫生

背景情况:

大肠杆菌是导致人类和牲畜感染的主要病原体.
大肠杆菌中的病毒性因素和抗菌素耐药性 (AMR) 引发了严重的公共卫生问题.
了解特定大肠杆菌克隆的流行病学对于有效控制至关重要.

研究的目的:

在食品和人类感染中识别和描述国际公认的大肠杆菌克隆.
研究不同大肠杆菌序列类型 (ST) 中抗生素耐药性基因和病毒性相关基因的流行情况.
评估与商业肉类中的抗生素耐药大肠杆菌相关的潜在风险.

主要方法:

从肉,猪肉和人类感染样本中检测和测序大肠杆菌分离物.
序列类型 (ST) 用于识别特定的大肠杆菌克隆.
基因组分析以确定抗生素耐药性基因 (例如,GyrA突变,CTX-M基因) 和病毒性相关基因 (VAGs).

主要成果:

在人类样本中确定了大肠杆菌克隆ST131 (分类B2) 占主导地位,在肉样本中占主导地位的ST38 (分类D).
观察到的最常见的抵抗机制是 gyrA 基因突变.
CTX-M-55是最常见的扩展谱β-乳糖酶 (ESBL),CTX-M-2和CTX-M-15的流行率有所变化.
与病毒性相关的基因 (gad, iss) 经常被检测到,特别是在人类分离物中.

结论:

这项研究揭示了社区环境中抗生素耐药大肠杆菌的复杂流行病学.
特定的大肠杆菌克隆在食品和人类感染中表现出明显的流行模式.
这些发现强调了来自商业肉类来源的抗生素耐药大肠杆菌对公共卫生构成的潜在风险.

关键词:

这就是ESBL.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.抗微生物耐药性抗微生物耐药性族群群组 (phylogroups) 是一个族群群.序列类型序列类型与毒性相关的基因

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