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在菌生物膜中确定抗菌素敏感性的创新方法

B Tegner Jacobson¹, Jessica DeWit-Dibbert¹, Eli T Selong²

¹Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59718, USA.

|January 8, 2025

概括

在Mycoplasma bovis和Mycoplasma bovoculi中形成的生物膜增加了抗生素耐药性,挑战了传统的测试方法. 一种新的流动细胞计生/死抗微生物敏感性测试 (LD-AST) 准确评估生物膜中的耐药性.

科学领域:

兽医微生物学兽医微生物学
细菌病原体的产生
抗微生物耐药性抗微生物耐药性

背景情况:

菌质 spp. 菌质 spp. 的情况. 是重要的牛病原体,导致诸如牛呼吸系统疾病综合体等疾病.
由Mycoplasma spp.形成的生物膜. 与抗生素耐药性增加有关,使敏感性测试复杂化.
目前的代谢pH依赖的变色方法对于Mycoplasma生物膜是不可靠的,因为代谢减少.

研究的目的:

开发和验证一种基于流细胞计的新方法,即活/死抗微生物敏感性测试 (LD-AST),用于准确测试生物膜形成的Mycoplasma中的抗微生物敏感性.
评估生物膜生长对Mycoplasma bovis和Mycoplasma bovoculi对 جنت米辛,恩洛夫洛克萨和四环素的抗菌敏感性的影响.

主要方法:

开发和优化基于流细胞计的活/死抗菌素敏感性测试 (LD-AST).
在浮游生物和生物膜条件下培养四种Mycoplasma菌株 (两个Mycoplasma bovis,两个Mycoplasma bovoculi).
使用LD-AST在暴露于 جنتamicin,enrofloxacin和tetracycline后确定最小杀菌度 (MBC).

主要成果:

所有的Mycoplasma菌株都像浮游生物细胞一样对抗生素敏感.
在新附着的生物膜中,四分之三的菌株对所有测试的抗生素都表现出完全的耐药性.
成熟的生物膜显示出可变的耐药性模式,一些菌株表现出增加或减少的耐药性,这取决于抗生素.

结论:

生物膜的形成显著增强了Mycoplasma bovis和Mycoplasma bovoculi中的抗菌耐药性.
LD-AST方法提供了一种强大而准确的方法,用于确定浮游生物和生物膜形成的菌体中的抗菌素敏感性.
这些发现突出了由于生物膜相关抗生素耐药性的原因,在牛群中治疗Mycoplasma感染的挑战.

关键词:

菌质玻尿素 (Mycoplasma bovis) 是一种菌体 sp. 菌体 sp. 菌体 sp. 菌体 sp.抗微生物耐药性抗微生物耐药性测试开发测试开发生物膜的形成.流动细胞计量是流动细胞计量的方法.活体/死体染色方式

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