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格拉姆阴性生物膜:结构和功能对抗生素加载混合聚合物菌根破坏的反应

Tsvetozara Damyanova¹, Rumena Stancheva², Milena N Leseva³

¹Department of Microbiology, Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, bl. 26, 1113 Sofia, Bulgaria.

|January 8, 2025

概括

新的混合聚合物微粒有效地破坏细菌生物膜,提供抗生素来对抗慢性感染. 这些药物输送系统对治疗具有挑战性的生物膜相关疾病充满希望.

科学领域:

生物材料科学生物材料科学
微生物学微生物学
药物输送系统药物输送系统

背景情况:

细菌生物膜是慢性感染的一个主要挑战,通过细胞外基质 (ECM) 保护细菌.
有效的治疗需要破坏生物膜的药物,具有抗菌活性,并可以透到ECM.
当前的疗法往往与生物膜的保护性质作斗争.

研究的目的:

开发能够破坏生物膜ECM和输送抗生素的新型混合聚合物微粒 (MPM).
给MPM加载西普罗夫洛克萨或阿齐思罗迈,以提高抗菌膜的疗效.
评估这些MPM的抗菌膜活性和药物输送能力,以对抗大肠杆菌和空气菌.

主要方法:

混合聚合物微粒 (MPM) 的合成,使用阴离子 (PDMAEMA-PCL-PDMAEMA) 和非离子 (PEO-PPO-PEO) 三块共聚物.
装载MPM与西普罗夫洛克萨或阿齐思罗米.
在预制的*E. coli*和*P. aeruginosa*生物膜上应用MPM.
使用扫描电子显微镜和ex vivo皮肤模型评估生物膜破坏,细菌活力和宿主组织相互作用.

主要成果:

MPM 显示出显著的生物膜破坏能力.
活力实验证实了通过被破坏的生物膜成功输送药物.
观察到细菌细胞改变和宿主免疫反应 (氧化,IL-17A) 的明显模式.
MPM对实验室菌株和临床隔离物都表现出有效性.

结论:

开发的MPM是有效的生物膜-ECM破坏性药物递送系统.
这些MPM为治疗与生物膜相关的慢性细菌感染提供了有希望的策略.
这项研究强调了定制的聚合物微粒在先进的治疗应用中的潜力.

关键词:

生物相容性生物相容性生物膜的破坏化聚合物是一种化聚合物.药物输送是药物输送的过程.一个 ex vivo 皮肤模型.细胞外囊泡中的细胞外囊泡.混合聚合物米塞尔的混合聚合物这些纳米管是纳米管.

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