PS-MPs诱导了肝脏炎症信号通路的炎症和酸化
Mengchao Ying1,2, Naimin Shao1,2, Cheng Dong1,2
1Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China.
Toxics
|January 8, 2025
概括
聚烯微塑料 (PS-MPs) 在小鼠和人类肝细胞中引发肝炎. 暴露会激活关键的炎症通路,表明这些新出现的污染物对健康的潜在风险.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 微塑料 (MP) 是一种新兴的污染物,广泛存在于环境中.
- 人们对MP的毒理影响,特别是对人类健康的影响,仍然不完全理解.
- 由于潜在的健康影响,对MP的肝脏暴露越来越令人担忧.
研究的目的:
- 研究0.1微米聚烯微塑料 (PS-MPs) 对小鼠和人类肝脏细胞系的炎症作用.
- 为了阐明背后的分子机制PS-MP诱导的肝炎.
- 评估PS-MPs对肝功能和细胞完整性的影响.
主要方法:
- 对小鼠和肝细胞系 (HL7702,HepG2) 暴露于不同度的PS-MPs.
- 分析小鼠肝脏指数,AST/ALT水平和乳酸脱酶 (LDH) 泄漏情况.
- 细胞活性的评估,炎症因子表达 (GM-CSF,IL-6,IL-8,IL-12p70) 和蛋白质酸化 (NF-κB,STATs) 在体外.
- 对托尔类受体4 (TLR4) /MyD88/NF-κB和JAK-STAT信号通路的研究.
主要成果:
- 在小鼠中,PS-MP暴露导致肝指数下降和AST/ALT值升高.
- 在体外,PS-MPs降低了肝细胞活力,并以剂量依赖的方式增加了LDH泄漏.
- 观察到炎症化学因子 (GM-CSF,IL-6,IL-8,IL-12p70) 的显著上调.
- PS-MPs激活了TLR4/MyD88/NF-κB和JAK-STAT信号通路,主要蛋白质的表达和酸化增加表明了这一点.
结论:
- 接触聚烯微塑料会刺激肝脏的炎症.
- 在肝细胞中,PS-MPs激活TLR4/MyD88/NF-κB和JAK-STAT信号通路.
- 这些发现强调了微塑料的潜在肝毒性,并要求进一步调查其健康风险.
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