冷保存协议和相关的超结构变化在休眠的芽的Vitis amurensis
Dan Sun1, Peijin Ni1, Jian Liu1
1College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
Plants (Basel, Switzerland)
|January 8, 2025
概括
在像Vitis amurensis这样的木质植物中冷保存休眠芽对于遗传资源的保护至关重要. CP3协议与特定的干燥和解方法相结合,显著提高了长期储存的芽生长能力.
科学领域:
- 植物科学 植物科学
- 低温生物学 低温生物学
- 园艺园艺 园艺园艺
背景情况:
- 保护木质植物,特别是果树的遗传资源至关重要.
- 休眠芽的冷保存提供了一种节省时间和成本的有效解决方案.
- 维蒂斯·阿穆伦西斯 (Vitis amurensis) 是一种重要的树木果物种,需要保护策略.
研究的目的:
- 评估和优化Vitis amurensis的休眠芽的冷保存协议.
- 为了确定V. amurensis芽的最佳条件干燥,冷和解.
- 评估冷保存的长期可行性和适用性,以保护遗传资源.
主要方法:
- 在三种V. amurensis品种的休眠芽上测试了三种冷保存协议.
- 在-5°C下进行28-30天的优化干燥,以达到25-30%的水分含量.
- 使用CP3协议与纸包装和24小时冷水解方法.
主要成果:
- 在CP3协议实现了66.67%的生存率,当芽被包装在纸袋.
- 最佳的干燥 (28-30天) 导致芽生长率达到45%.
- 1月份收集的芽显示了冷保存后的最高可行性,而11月和3月的收集是最低的.
- 在2019-2021年期间",Shuanghong",Zuoshanyi"和"Shuangfeng"品种的平均生存率超过了60%.
- 内部芽细胞保留了生命力,在解后恢复了生长,尽管外部细胞受损.
结论:
- CP3冷保存协议,优化了干燥和解,对Vitis amurensis.有效.
- 这种方法显著提高了休眠芽的生存能力,以保护遗传资源.
- 开发的协议适用于研究的V. amurensis品种,确保遗传物质的保存.
更多相关视频
07:25Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
Published on: March 20, 2019
6.2K
06:53Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
5.1K
相关概念视频
Cryo-electron Microscopy
3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K
Responses to Heat and Cold Stress
13.3K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.3K
