用添加的类似钻石的涂层,用于长期增强电聚 ((ε-caprolactone) 基架表面的细胞粘附
Semen Goreninskii1, Yuri Yuriev2,3, Artem Runts3
1Additive Technologies Center, Tomsk Polytechnic University, Tomsk 634050, Russia.
Polymers
|January 8, 2025
概括
用添加的钻石状碳 (N-DLC) 涂层增强了用于组织工程的聚乙烯 (PCL) 支架. 这些N-DLC涂层的PCL支架显示了细胞粘附和增殖的改善,即使经过六个月的储存.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 表面工程是什么?表面工程是什么?
背景情况:
- 在组织工程中,电聚-ε-caprolactone (PCL) 支架至关重要,但其细胞粘附性较差.
- 已知用添加的钻石状碳 (N-DLC) 涂层可以提高植入物生物相容性和功能.
研究的目的:
- 使用脉冲真空弧沉积 (PVAD) 在电PCL支架上制造和表征N-DLC涂层.
- 研究N-DLC沉积过程中不同气压力对PCL支架性能的影响.
- 评估N-DLC涂层对细胞粘附,增殖和长期稳定性的影响.
主要方法:
- 在PCL支架上制造N-DLC涂层,采用不同压的脉冲真空弧沉积 (PVAD).
- 脚手架形态,机械强度和物理化学性质的表征.
- 在涂层支架上评估人间介质干细胞 (MMSC) 粘附和增殖.
- 在储存6个月后评估涂层稳定性和细胞粘附性.
主要成果:
- 随着气压力的增加,N-DLC涂层沉积增加了含量高达10倍,表面粗度增加了2倍.
- 经过修改的PCL支架显著增强了人类介质干细胞 (MMSCs) 的粘附和扩散.
- 在N-DLC涂层的PCL支架上,在环境条件下储存6个月后,MMSC的附着性得到了改善.
结论:
- PVAD是一种有效的技术,用于将N-DLC涂层应用于电PCL支架.
- 通过改善细胞粘附和增殖,N-DLC涂层显著提高PCL支架的生物性能.
- N-DLC涂层的稳定性确保了用于组织工程应用的修改PCL支架的长期有效性.
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