相关实验视频

Updated: Jun 3, 2025

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

表观遗传偏移与HBV疫苗接种的有效性有关

Francesca Ferraresi¹, Simona Anticoli², Stefano Salvioli^3,4

¹Department of Chemistry "Giacomo Ciamician", University of Bologna, 40126 Bologna, Italy.

|January 8, 2025

概括

表观遗传变化或表观变异在那些不对乙型肝炎病毒 (HBV) 疫苗有反应的人群中积累起来. 这种与年龄相关的表观遗传漂移可能解释了疫苗不反应.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
疫苗学疫苗学疫苗学

背景情况:

乙型肝炎病毒 (HBV) 感染导致严重的肝病死亡率.
虽然HBV疫苗是有效的,但10%的人无法产生足够的免疫力.
了解疫苗反应机制对于公共卫生至关重要.

研究的目的:

为了研究B细胞中的表观遗传漂移,从对HBV疫苗有反应和没有反应的个体.
为了确定与HBV疫苗不响应相关的特定表观遗传变化.

主要方法:

通过计算罕见的DNA甲基化变体 (epivariants) 来量化表观遗传漂移.
分析了41名HBV疫苗响应者和30名不响应者的表观基因组数据.
分析包括识别丰富的染色体和基因,途径分析,以及与表观遗传钟和的相关性.

主要成果:

非响应者 (NRs) 显示出经变体的累积,特别是超甲基化异常.
特定的染色体 (1,3,11,12,14) 和基因 (例如,RUSC1_AS1,TROVE2) 在NRs中被丰富了经变异.
与年龄相关的表观遗传钟和男性的DNA甲基化相关的经变负荷.

结论:

与年龄相关的表观遗传障碍有助于对HBV疫苗的不反应.
观察到表观遗传漂移,时钟和之间的相互联系,尽管与改变的免疫活性没有直接联系.

关键词:

B 细胞 B 细胞 B 细胞表观遗传衰老表观遗传衰老表观遗传学是指表观遗传学.这种类型的变体.乙型肝炎 B 型肝炎免疫反应的免疫反应.疫苗疫苗疫苗疫苗

更多相关视频

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

相关概念视频

Genetic Drift

Genetic Drift

Natural selection—probably the most well-known evolutionary mechanism—increases the prevalence of traits that enhance survival and reproduction. However, evolution does not merely propagate favorable traits, nor does it always benefit populations.

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Mutation, Gene Flow, and Genetic Drift

Mutation, Gene Flow, and Genetic Drift

In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).

Vaccinations

Vaccinations

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.