基于细菌的检测方法的非类型型型沙门氏菌在食品中的有效性:系统性审查
Preeda Phothaworn1,2, Chatruthai Meethai2, Wanchat Sirisarn3
1Center of Excellence Research for Melioidosis and Microorganisms (CERMM), Walailak University, Nakhon Si Thammarat 80160, Thailand.
Viruses
|January 8, 2025
概括
菌体可以快速,灵敏地检测食品中的非类风型沙门氏菌 (NTS),克服传统方法的局限性. 这种基于菌体的方法通过更快地识别细菌污染来提高食品安全.
科学领域:
- 食品安全和微生物学
- 菌体的应用 菌体应用
- 诊断试验的发展.
背景情况:
- 非伤寒型沙门氏菌 (NTS) 污染对公众健康构成重大风险.
- 传统的NTS检测方法缓慢且劳动密集,阻碍了有效的食品安全监督.
- 细菌菌体 (菌体),具有其宿主特异性,为快速检测细菌提供了一个有希望的替代方案.
研究的目的:
- 审查和分析细菌菌体的应用,以快速检测食品中的非类风性沙门氏菌.
- 与传统技术相比,评估基于菌体的检测方法的灵敏度,特异性和速度.
- 确定在食品安全方面优化菌体基NTS检测的挑战和未来方向.
主要方法:
- 对食品中基于菌体检测NTS研究的系统文献综述.
- 数据提取和使用JBI批判性评估检查清单对选定的文章进行质量评估.
- 分析各种基于菌体的检测策略,包括与免疫试验和光谱学的整合.
主要成果:
- 基于菌体的测试实现了快速和高度敏感的NTS检测,在30分钟内极限低至7-8CFU/mL.
- 将菌体与侧流免疫试验,SERS,光和电化学等技术集成,提高了检测能力.
- 审查的研究针对沙门氏菌的丰富,快速检测和有效的恢复在多种食物矩阵.
结论:
- 菌体具有显著的潜力,可以作为一种快速和灵敏的工具来检测食品中的非伤寒型沙门氏菌.
- 基于菌体的生物感知平台为传统诊断方法提供了可行的替代方案或补充,提高了食品安全.
- 进一步的研究和开发至关重要,以克服现有的挑战,并充分实施基于菌体的检测,以加强食品安全协议.
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