玉米串状病毒:单一和双胞胎囊架构
Antonette Bennett1, Joshua A Hull1, Mario Mietzsch1
1Department of Biochemistry and Molecular Biology, College of Medicine Center for Structural Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0245, USA.
Viruses
|January 8, 2025
概括
双子病毒是重要的植物病原体,有两个状结构. 这项研究揭示了它们的高分辨率冷EM结构,详细介绍了外衣蛋白相互作用,并提出了抗病毒点.
科学领域:
- 结构病毒学 结构病毒学
- 植物病理学 植物病理学
- 生物物理学的生物物理.
背景情况:
- 双子病毒是单链DNA (ssDNA) 植物病毒,具有重要的农业和经济影响.
- 它们的头体呈现出两种独特的建筑形式:T = 1 二元面体和双胞胎半同面体.
研究的目的:
- 使用冷电子显微镜 (cryo-EM) 确定玉米病毒的两种状形式的高分辨率结构.
- 分析皮层蛋白 (CP) 和控制囊组装的CP基因组相互作用.
- 为Geminiviridae提供一个结构图谱,并确定潜在的抗病毒点.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 来解析这些结构.
- 对Geminiviridae成员进行比较的结构和序列分析.
主要成果:
- 获得了T = 1的高分辨率结构,包括T = 1的二元面体和双胞胎准同位体的玉米菌病毒体.
- 对外衣蛋白 (CP) 和CP基因组相互作用的详细见解得到了阐明.
- 在Geminiviridae成员 (60-95%) 中观察到结构性保护,与较低的序列相似性 (21-30%) 相比.
结论:
- 这项研究提供了对双子病毒囊组合的全面结构理解.
- 鉴定的结构性保护表明潜在的广泛的抗病毒策略.
- 这些发现突出了针对抗病毒开发的双子病毒囊体的目标区域.
相关概念视频
00:56Viral Structure
61.7K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
61.7K
00:50What are Viruses?
113.7K
Overview
113.7K
02:41Protein Complex Assembly
10.5K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.5K
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
01:32Pinching-off of Coated Vesicles
3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K
01:10Retrovirus Life Cycles
45.6K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.6K