scTRIM44 通过RIG-I和MDA5-介导的干扰素信号传递积极调节的Siniperca Chuatsi Rhabdovirus
Yinjie Niu1, Xinmei Yang1, Hongru Liang1
1Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Key Laboratory of Fishery Drug Development, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Viruses
|January 8, 2025
概括
西尼珀卡西TRIM44 (scTRIM44) 通过增强干扰素信号传递,促进西尼珀卡西拉博病毒 (SCRV) 感染. 然而,scTRIM44不会影响感染性脏和亡病毒 (ISKNV) 或大鱼病毒 (LMBV) 感染.
科学领域:
- 水产养殖和鱼类病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 三方基因含有44 (TRIM44) 与包括病毒感染在内的各种疾病有关.
- 在鱼类中TRIM44的特定作用,特别是TRIM44 (scTRIM44),在病毒发病过程中在很大程度上是未知的.
研究的目的:
- 为了研究scTRIM44.4的分子特性.
- 阐明scTRIM44的功能,以应对感染性脏和脏亡病毒 (ISKNV),大鱼病毒 (LMBV) 和Siniperca chuatsi rhabdovirus (SCRV) 的感染.
主要方法:
- 对scTRIM44分子结构的分析,包括域识别 (B-box,coil-coil).
- 在免疫组织和病毒挑战后,对scTRIM44mRNA表达的定量评估.
- 在细胞中操纵scTRIM44表达 (过度表达和淘汰),以评估其对病毒复制的影响.
- 研究scTRIM44在干扰素信号通路中的作用 (RIG-I,MDA5).
主要成果:
- ScTRIM44拥有B-box和coiled-coil域,但没有正规的RING域.
- 在免疫组织中,scTRIM44 mRNA的表达高,在ISKNV和LMBV感染时下降,但在SCRV感染时增加.
- ScTRIM44积极调节SCRV感染和RIG-I/MDA5介导的干扰素信号,但没有显著影响ISKNV或LMBV复制.
结论:
- 在Siniperca chuatsi*中,ScTRIM44在抗病毒反应中起着差异性作用.
- ScTRIM44通过调节干扰素信号通路来促进SCRV感染.
- 在ISKNV和LMBV感染中,ScTRIM44的参与是可以忽略不计的,这表明病毒特异性相互作用.
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