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使用成像生成和表征鼻上皮模型的协议,用于呼吸道病毒.

Victor H K Lam1, Aleena Ghafoor1, Yazan Khan1

  • 1Department of Microbiology and Immunology, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada.

STAR protocols
|January 8, 2025
PubMed
概括

这项研究详细介绍了使用空气-液体接口 (ALI) 培养用于严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) 研究的鼻上皮细胞培养的协议. 该方法解决了成像,粘液检测和体外病毒定量方面的挑战.

关键词:
细胞培养培养的细胞培养.细胞隔离细胞隔离微生物学的微生物.显微镜 显微镜是指使用显微镜.

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Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...