重组猪FGF1促进肌肉干细胞增殖和线粒体功能,用于培养肉类生产
Qingying Liu1,2, Lianghua Xie1,2, Wei Chen1,2
1Department of Traditional Chinese Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Journal of agricultural and food chemistry
|January 8, 2025
概括
研究人员开发了一种生产用于培养肉类的重组猪纤维细胞生长因子1 (rpFGF1) 的方法. 这种rpFGF1在无血清条件下增强肌肉干细胞的增殖,有助于可持续的肉类生产.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 食品科学 食品科学 食品科学
背景情况:
- 培养肉类生产需要有效的,没有动物成分的肌肉干细胞 (MuSC) 扩张体外系统.
- 纤维细胞生长因子1 (FGF1) 对于调节 MuSC 功能至关重要.
研究的目的:
- 建立一种生产重组猪FGF1 (rpFGF1) 的高效方法.
- 在无血清条件下研究rpFGF1对猪细胞增殖和线粒体功能的影响.
主要方法:
- 在大肠杆菌中,rpFGF1的可溶性表达和净化.
- 在无血清条件下使用rpFGF1治疗猪肌肉细胞.
- 对MuSC增殖,线粒体裂变,线粒体和ERK依赖的DRP1酸化的分析.
主要成果:
- 实现了48mg/L的纯化rpFGF1.1的产量.
- rpFGF1在无血清介质中显著增强了猪的MuSC增殖.
- rpFGF1通过ERK依赖的DRP1酸化促进了线粒体分裂和线粒体衰变,改善了线粒体功能和增殖能力.
结论:
- 在无血清条件下,rpFGF1有效地促进了猪细胞的增殖和功能.
- rpFGF1具有开发可扩展和可持续的无血清媒介的潜力,用于培养肉类生产.
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