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小RNA CjNC110调节激活的甲基循环,使可以通过Campylobacter jejuni的最佳殖

Brandon Ruddell^1,2, Alan Hassall^1,2, Orhan Sahin^2,3

¹Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.

|January 8, 2025

概括

小RNA CjNC110调节了Campylobacter jejuni中的L-氨酸生产,影响了的殖民. 活性甲基循环中的菌株特异性影响殖民成功,突出显示CjNC110

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

非编码小RNA (sRNA) 对于肠道病原体的生存至关重要.
动物传染病原体Campylobacter jejuni依赖sRNAs进行殖民和生存.

研究的目的:

研究C. jejuni.中sRNA CjNC110的调节作用.
确定CjNC110如何影响殖和L-氨酸 (L-met) 水平.
探索CjNC110介导调节中的菌株特定变异.

主要方法:

使用的C. jejuni菌株是IA3902和W7.
为sRNA CjNC110和metAB基因产生了删除突变.
在中评估了细菌殖民.
量化细胞内L-氨酸水平.

主要成果:

在这两种菌株中,CjNC110调节了运动性和自身聚集.
在IA3902中,CjNC110的删除减少了的殖民,但不是W7.
活性甲基循环 (AMC) 功能的菌株特异性差异,由于MetAB的存在,影响了L-met水平和殖民.
在IA3902中恢复metAB或在W7调节的L-met级别和殖民中删除它.

结论:

在C. jejuni.中,CjNC110对AMC和L-met可用性的调节是保留的.
通过AMC生成L-met对于最佳的C. jejuni宿主殖民至关重要.
菌株特定的基因组内容可以掩盖或改变保存的sRNAs的表型效应.

关键词:

这是坎比洛巴克特菌.L-甲氨酸是一种L-氨酸.激活的甲基循环.的殖民化殖民化很小的RNAs是小的RNAs.

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