Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

CT reconstruction using diffusion posterior sampling conditioned on a nonlinear measurement model.

Journal of medical imaging (Bellingham, Wash.)·2024
Same author

Super-resolution diffractive neural network for all-optical direction of arrival estimation beyond diffraction limits.

Light, science & applications·2024
Same author

Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue.

Nature biomedical engineering·2023
Same author

Structure of the NuA4 acetyltransferase complex bound to the nucleosome.

Nature·2022
Same author

In situ structure of intestinal apical surface reveals nanobristles on microvilli.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

EPicker is an exemplar-based continual learning approach for knowledge accumulation in cryoEM particle picking.

Nature communications·2022

相关实验视频

Updated: Jun 3, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.2K

MPicker:可视化和挑选膜蛋白用于冷电子断层扫描.

Xiaofeng Yan1,2,3,4,5, Shudong Li5,6, Weilin Huang1,2,3,4,5,6,7

  • 1Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing, China.

Nature communications
|January 8, 2025
PubMed
概括

一种新的膜平方法和MPicker软件增强了冷电子断层扫描 (cryoET) 数据集中的膜蛋白的可视化. 这种方法简化了在充满挑战的细胞环境中的in situ蛋白质结构的分析.

更多相关视频

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
10:39

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

29.8K
Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data
07:17

Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data

Published on: January 24, 2025

804

相关实验视频

Last Updated: Jun 3, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.2K
Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
10:39

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

29.8K
Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data
07:17

Leveraging Virtual Reality for Immersive Segmentation and Analysis of Cryo-Electron Tomography Data

Published on: January 24, 2025

804

科学领域:

  • 结构生物学 结构生物学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 低温电子断层扫描 (cryoET) 可以在现场确定宏分子结构.
  • 在它们的原生细胞环境中研究膜蛋白是至关重要的,但由于曲和对比问题在cryoET中具有挑战性.
  • 这些因素阻碍了膜蛋白结构的可视化和分析.

研究的目的:

  • 开发一种膜平面化方法,以改善冷ET中的膜蛋白的可视化.
  • 引入MPicker软件用于膜蛋白的自动可视化,定位和定向确定.
  • 为了促进膜蛋白的子断层图像平均值.

主要方法:

  • 开发了一种新的膜平技术,以纠正膜曲率.
  • 创建了MPicker软件,集成了自动粒子采集和粗对齐算法.
  • 该方法和软件在各种细胞类型的冷ET断层图像上进行了测试.

主要成果:

  • 膜平方法有效地减少了空间复杂性,并改善了膜蛋白的可视化.
  • MPicker软件证明了膜蛋白的成功自动挑选和对齐.
  • 综合方法增强了 in situ 膜蛋白结构的分析.

结论:

  • 开发的膜平方法和MPicker软件显著推进了使用cryoET.ET进行膜蛋白的in situ结构分析.
  • 这种方法克服了与膜曲率和信号掩盖相关的关键挑战.
  • 它为了解细胞环境中的膜蛋白功能和相互作用提供了有价值的工具.