彩虹鱼 (Oncorhynchus mykiss) 对急性热应激的高耐性和低耐性的基因表达的差异
Leah A Turner1, Anne A Easton1,2, Moira M Ferguson1
1Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada.
PloS one
|January 8, 2025
概括
彩虹鱼具有高耐热性,抑制生长并增强修复途径. 耐受性较低的鱼优先考虑发育和新陈代谢,这表明它们对热应激有不同的适应策略.
科学领域:
- 在ectotherms的环境适应.
- 热耐受性的分子机制
- 气候变化对水生生物的影响
背景情况:
- 全球气温上升对外热物种构成重大威胁.
- 了解耐热度变化对于预测物种生存至关重要.
- 彩虹鱼 (Oncorhynchus mykiss) 作为研究热应激反应的模型.
研究的目的:
- 为了确定高和低耐热彩虹鱼之间的分子和生理差异.
- 阐明与急性热应激适应相关的基因表达模式.
- 提供关于减轻鱼类气候变化影响的机制的见解.
主要方法:
- 利用临界热最大试验来区分彩虹鱼株的高和低热耐受性.
- 分析了肝脏转录组概况,以确定差异性基因表达.
- 在高耐受性,低耐受性和对照组之间比较基因表达模式.
主要成果:
- 高耐受性鱼抑制了与生长相关的功能,并增强了自,神经退行过程调节和与压力相关的蛋白质合成 (包括结合体活动和mRNA调节).
- 耐受性较低的鱼表现出更高的转录多样性,具有较高的发育,细胞骨,形态,脂质和碳水化合物代谢过程.
- 观察到经典的热冲击蛋白反应,但没有区分耐受性水平.
结论:
- 彩虹鱼的高热耐受性与防止损害和增强修复途径有关.
- 低热耐受性与细胞更换和代谢过程有关.
- 这些发现为鱼类适应热应激的适应策略提供了更深入的理解.
相关概念视频
Background and Environment Affect Phenotype
6.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.4K
Responses to Heat and Cold Stress
13.3K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.3K
Osmoregulation in Fishes
49.3K
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
49.3K


