相关实验视频

Updated: Jun 3, 2025

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

在耐药品种"UI Silver"中识别和验证矮型的两个定量特征位点

Pabitra Joshi¹, Guriqbal Singh Dhillon¹, Yaotian Gao¹

¹Department of Plant Sciences, University of Idaho Aberdeen, R and E Center, Aberdeen, ID, 83210, USA.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|January 8, 2025

概括

研究人员在6D染色体上确定了两个主要的定量特征位点 (QTLs),用于提高小麦的矮人抗性. 开发的KASP标记物将通过使用标记物辅助选择加速这种真菌病的繁殖.

科学领域:

植物育种与遗传学
农业科学农业科学
植物病理学植物病理学

背景情况:

由Tilletia controversa引起的矮,是小麦中的破坏性真菌病,导致大量的产量和质量损失.
寻求矮人抗性的传统育种是耗时的,需要进行广泛的实地试验和晚期疾病评估.
分子标记器辅助选择 (MAS) 是提高小麦品种抗病能力的更有效的策略.

研究的目的:

识别和绘制与小麦中矮抗性相关的定量特征位点 (QTL).
开发和验证竞争性等位基特异性PCR (KASP) 标记器,用于对矮体抗性进行标记器辅助选择 (MAS).
评估在育种计划中识别的QTL和标记的有用性,以开发耐药冬季小麦品种.

主要方法:

从一种耐药 (UI Silver) 和易受 (Shaan89150) 的小麦交叉中,一个双倍的平分体 (DH) 映射群体 (135条线) 被表型化为矮人抗性.
用Illumina 90K SNP iSelect标记平台进行基因型鉴定.
使用包括性复合间隔映射 (ICIM) 识别QTL,随后在多样化的冬季小麦面板中开发和验证KASP标志物.

主要成果:

两种显著的QTLs,染色体臂6DL上的Qdb.ssdhui-6DL和6DS上的Qdb.ssdhui-6DS被一致确定,分别解释了15.2935.40%和~11%的表型变异.
这些QTLs显示了添加的添加效应,有助于增强矮人抗性.
开发的KASP标记被验证,证实了它们与区域适应的冬季小麦品种中耐药性的关联.

结论:

在6D染色体上发现的QTLs为改善小麦中矮体抗性提供了宝贵的遗传资源.
品种"UI Silver"拥有这些耐药性QTL,并作为未来育种工作的关键母体.
经过验证的KASP标记器促进了有效的标记器辅助选择,加速了抗病小麦品种的发展.

更多相关视频

Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce Lactuca sativa Germplasm Collections

Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce Lactuca sativa Germplasm Collections

Published on: April 17, 2015

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

相关概念视频

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

Dihybrid Crosses

Dihybrid Crosses

Chi-square Analysis

Chi-square Analysis

The chi-square test is a statistical hypothesis test. It is used to check whether there is a significant difference between an expected value and an observed value. In the context of genetics, it enables us to either accept or reject a hypothesis, based on how much the observed values deviate from the expected values.
The chi-square test was developed by Pearson in 1990.
The first step of performing a Chi-square analysis is to establish a null hypothesis, which assumes that there is no real...

Monohybrid Crosses

Monohybrid Crosses