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SARS-CoV-2的环境传播:使用Crassphage和下一代测序协议进行分析

André Vinicius Costa Ribeiro^1,2, Camille Ferreira Mannarino³, Thiago Dos Santos Leal⁴

¹Stricto Sensu Graduate Program in Cellular and Molecular Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, 21040-360, Brazil. andre.ribeiro@fiocruz.br.

Food and environmental virology

|January 8, 2025

概括

在COVID-19大流行期间,严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 在里约热内卢的废水和溪水中被检测到. 交叉组装菌体 (crAssphage) 证实了便污染,全基因组测序确定了玛变种.

科学领域:

环境微生物学环境微生物学
病毒学病毒学
公共卫生公共卫生

背景情况:

污水监测对于监测COVID-19等传染病至关重要.
了解病原体在水体中的传播,有助于制定公共卫生战略.
像crAssphage这样的生物标志物有助于评估环境样本中的便污染.

研究的目的:

在COVID-19大流行期间调查废水和地表水中SARS-CoV-2的存在和流行情况.
使用crAssphage作为便指标来评估水污染.
用下一代测序来识别在环境中传播的SARS-CoV-2变种.

主要方法:

在里约热内卢收集原始废水和地表水样本.
使用超离心和膜过的病毒度.
通过RT-qPCR检测和定量SARS-CoV-2和crAssphage.
全基因组测序用于变种识别.

主要成果:

在92.5%的废水和31.25%的溪水样本中检测到SARS-CoV-2RNA;在海水中不存在.
克拉斯巴格证实了广泛的便污染,特别是在溪水中.
在SARS-CoV-2水平和13天滞后的临床病例之间发现了中度至强度的相关性.
全基因组测序确定了SARS-CoV-2的玛变种.

结论:

废水和溪流水作为SARS-CoV-2传播的重要储水池.
CrAssphage是各种水类便污染的可靠指标.
环境监测,包括变种识别,补充了流行病监测的临床数据.

关键词:

这是下一代测序.这就是SARS-CoV-2病毒.海水是海水,海水是海水.溪流的水流水的水废水是废水,废水就是废水.在Crassphage中使用.

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