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冠状素硫酸和葡萄糖胺硫酸作为保护性和抗炎剂在瘤性结肠炎中 DSS模型在老鼠中
Luiz Gustavo de Oliveira1, Arthur Girardi Carpanez1, João Victor Gerheim da Silva1
1Instituto de Ciências Biológicas da Universidade Federal de Juiz de Fora, Laboratório de Análises de Glicoconjugados, Departamento de Bioquímica, Juiz de Fora, MG, Brasil.
Arquivos de gastroenterologia
|January 8, 2025
概括
二甲酸盐 (CS) 和葡萄糖胺 (GlcN) 降低肠道炎症,并保护肠道屏障,在性结肠炎的老鼠模型中. 这种组合疗法在治疗炎症性肠病 (IBD) 中表现有前途.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
背景情况:
- 氏丁硫酸盐 (CS) 和葡萄糖胺 (GlcN) 已知具有抗炎性质,特别是在减少金属蛋白酶 (MMP) 和炎症介质方面.
- 性结肠炎 (UC) 是一种慢性炎症性肠病 (IBD),其特点是肠道炎症和屏障功能障碍.
研究的目的:
- 为了研究CS的结构.
- 评估CS/GlcN在硫酸 (DSS) 诱导的性结肠炎的老鼠模型中的抗炎和保护作用.
主要方法:
- CS结构和分子重量的表征.
- 在DSS诱导的大肠炎的老鼠中,口服CS/GlcN.
- 评估组织学分数,杯细胞破坏,氧化 (NO) 生产,骨髓氧化酶 (MPO) 活性和MMP活性 (特别是MMP-9).
- 对CS/GlcN在肠道上皮细胞上的细胞毒性的评估.
主要成果:
- CS分糖化合物的组成与C4S标准相似,其模态分子量为30.4kDa.
- 口服CS/GlcN显著改善了急性结肠炎的严重程度,降低了组织学分数和杯状细胞破坏.
- 观察到NO产量,MPO活性和MMP活性 (特别是MMP-9) 的下降.
- CS/GlcN在肠道上皮细胞中没有表现出细胞毒性.
结论:
- 在大肠炎的老鼠模型中,CS/GlcN组合治疗有效降低了肠道炎症,并保护了肠道屏障.
- 这些发现表明,CS/GlcN可能是炎症性肠病 (IBD) 的有益治疗选择.
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