与多种类型的Pseudomonadota家族有关联的磁性细菌生物矿化细胞内Ca-碳酸盐
Camille C Mangin1, Karim Benzerara2, Marine Bergot1
1Aix-Marseille Université, CNRS, CEA, BIAM, UMR7265 Institut de Biosciences and Biotechnologies d'Aix-Marseille, Cadarache research centre, F-13115 Saint-Paul-lez-Durance, France.
The ISME journal
|January 8, 2025
概括
细胞内碳酸的形成,先前与一种细菌属有关,现在在各种磁触性细菌中发现. 这种生物矿物化过程在细菌中很普遍,特别是在无氧环境中.
科学领域:
- 微生物学 微生物学
- 生物矿物化 生物矿物化
- 基因组学就是基因组学.
背景情况:
- 细胞内碳酸的形成主要与巨型Gammaproteobacteria属Achromatium有关.
- 最近的观察扩展到光合成的菌和磁性抗菌Alphaproteobacteria,形成无形碳酸和磁体.
- 在帕文湖之前的研究低估了产生细胞内无形碳酸的细菌的多样性.
研究的目的:
- 为了全面研究形成细胞内无形碳酸的磁触性细菌的多样性.
- 通过环境微生物学,基因组学和矿物学来描述这些细菌.
- 探索这种生物矿物化的代谢能力和进化影响.
主要方法:
- 通过微操作进行细胞分类.
- 环境微生物学,基因组学和矿物学分析.
- 扫描传输电子显微镜与能量分散式X射线光谱学.
- 冷软X射线断层扫描用于3D包含体积分析.
- 代谢网络建模.
主要成果:
- 鉴定出了两个类Pseudomonadota内的不同属的几种磁触性细菌物种.
- 所有描述的物种都形成细胞内无形碳酸,占细胞体积的44-68%.
- 代谢建模揭示了多种能力,包括甲基变和CO2固定通过阿尔法和玛蛋白质细菌的不同途径.
结论:
- 细菌中的细胞内碳生成显示出一个融合的进化模式.
- 在无氧环境中的二氧化碳固定促进了细胞内碳酸的形成.
- 这项研究显著扩大了已知的细菌多样性,这些细菌参与了这个生物矿物化过程.
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