单氨酸通过削弱NF-κBB来减弱细胞癌细胞进展和M2巨细胞极化
Heping Qiu1, Fei Liu1, Mei Qiu2
1Department of Urology, The Second Affiliated Hospital of Nanchang University, No.1, Minde Road, East Lake District, Nanchang, 330006, Jiangxi, China.
International urology and nephrology
|January 8, 2025
概括
单胺通过通过NF-κB通路减少细胞增殖和M2巨细胞极化,抑制细胞癌 (RCC) 的进展. 这种天然化合物显示出作为RCC的治疗剂的潜力.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 细胞癌 (RCC) 是一个重大的健康问题.
- 了解分子机制和瘤微环境相互作用对于有效的RCC治疗至关重要.
- 确定针对癌细胞及其周围环境的新型治疗剂是关键的研究领域.
研究的目的:
- 为了研究单二烯对细胞癌 (RCC) 的治疗效果.
- 阐明单素作用的基本机制,特别是其对NF-κB通路和M2巨细胞两极分化的影响.
- 评估单素作为RCC新型治疗剂的潜力.
主要方法:
- 细胞增殖,入侵和细胞亡测定 (CCK-8,Transwell,流细胞计) 在用单素治疗的RCC细胞上进行.
- 在共同培养系统中评估了巨分化,使用qRT-PCR,西斑和ELISA检测M2标记物和细胞因子.
- 在RCC小鼠异种移植模型中评估了单素的治疗疗效.
主要成果:
- 单素显著抑制了RCC细胞的增殖和入侵,同时促进了细胞亡.
- 单胺抑制了RCC细胞中NF-κB通路的激活,减少了关键的下游标,如Bcl-2,c-Myc和MMP9.
- 单胺降低了M2巨细胞极化标记物和细胞因子的调节,表明瘤微环境的调节. 兰克尔扭转了这些影响.
- 在体内研究证实,单氨酸减少了瘤生长,诱导了亡,并在RCC小鼠模型中抑制了NF-κB途径.
结论:
- 单胺有效地预防RCC的恶性进展.
- 单氨酸通过抑制NF-κB通路并减少M2巨细胞两极分化来发挥其抗癌作用.
- 单烯显示出作为RCC治疗剂的潜力,向瘤细胞和瘤微环境.
更多相关视频
06:38Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
Published on: August 2, 2021
27.1K
10:43Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
Published on: September 18, 2020
28.6K
相关概念视频
02:26NF-κB-dependent Signaling Pathway
7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.2K
03:03mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
02:23Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K