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在马达加斯加农村,De Novo使用便携式纳米孔测序在一个临灭绝的的基因组组装

Lindsey Hauff^1,2,3, Noa Elosmie Rasoanaivo⁴, Andriamahery Razafindrakoto⁵

¹Department of Ecology, Evolution, and Natural Resources Rutgers University New Brunswick New Jersey USA.

Ecology and evolution

|January 8, 2025

概括

研究人员在马达加斯加使用便携式纳米孔测序生成了第一个红额棕色 (Eulemur rufifrons) 参考基因组. 这一突破支持保护基因组学,并解决全球研究不平等问题.

科学领域:

基因组学就是基因组学.
保护生物学保护生物学
灵长类动物研究研究

背景情况:

类动物是面临高度威胁的哺乳动物,面临着人类造成的压力.
保护基因组学需要高质量的参考基因组来有效识别人口.
基因组研究往往涉及样本出口,造成全球准入和培训不平等.

研究的目的:

为临灭绝的红额棕 (Eulemur rufifrons) 提供第一个参考基因组.
用便携式技术在东道国内进行先进的基因组测序的可行性.
解决基因组研究获取方面的不平等问题,并建立当地能力.

主要方法:

在马达加斯加的一个存档的E. rufifrons标本上进行了长读纳米孔测序.
在中心ValBio研究站使用便携式牛津纳米孔MINION技术.
为基因组组装生成了超过750GB的测序数据.

主要成果:

组装了一个2.157Gb的核基因组,有2980个contigs和一个N50的101.6Mb.
组装了一个17,108 bp的线粒基因组.
获得了30倍的平均覆盖率和96.1%的BUSCO完整度得分,用于灵长类动物特异性基因.

结论:

生成的参考基因组是E. rufifrons的第一个,也是Eulemur属的唯一注释的基因组.
这种资源对于保护的基因组研究至关重要.
该研究强调了便携式测序的潜力,以促进研究公平,并在全球范围内建立基因组能力.

关键词:

中心ValBio研究站牛津纳米孔测序牛津纳米孔测序保护基因组学保护基因组学基因组组装组合的基因组.有红额的棕色鼠.参考基因组是指基因组中的基因组.

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