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通过光谱学和对接方法对苏丹染料与DNA的结合性相互作用进行全面的调查

Hongmei Zhang¹, Yunxia Yang¹, Yanqing Wang²

¹School of Chemical and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224007, People's Republic of China.

Journal of fluorescence

|January 8, 2025

概括

苏丹染料 (苏丹I-IV) 与小牛胸腺DNA (ct-DNA) 结合,主要是通过小沟相互作用. 增加的分子结合增强了结合亲和力,这对于理解食品安全和致癌机制至关重要.

科学领域:

分子生物学分子生物学
生物化学生物化学
食品安全分析食品安全分析

背景情况:

苏丹染料被公认为致癌物质,需要严格监测食品安全.
了解苏丹染料与DNA的分子相互作用,对于评估它们的安全性至关重要.
牛犊胸腺DNA (ct-DNA) 作为研究DNA-染料相互作用的模型.

研究的目的:

在分子水平上研究苏丹I-IV染料和小牛胸腺DNA (ct-DNA) 之间的结合相互作用.
为了阐明苏丹染料与DNA的结合方式和亲和力.
为了将苏丹染料的结构特征与它们的DNA结合能力相关联.

主要方法:

光谱技术包括同步光和紫外线光谱.
乙化 (EB) 和霍希斯特33258的排位测试.
分子建模和计算分析.

主要成果:

光谱数据表明苏丹I-IV和ct-DNA之间的复杂形成.
排位测试证实了苏丹染料与ct-DNA的小沟结合模式.
分子建模证实了实验结果,突出了芳香环和结合在结合中的作用.

结论:

苏丹I-IV染料与ct-DNA相互作用,主要是通过小沟结合.
苏丹染料中的分子结合程度影响了它们的DNA结合亲和力,苏丹III和IV显示出更强的相互作用.
这些发现为苏丹染料的潜在基因毒性提供了分子洞察力,这与食品安全法规有关.

关键词:

绑定模式绑定模式它们是DNA DNA DNA DNA.苏丹的染料是苏丹的染料

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