定量DNA元编码揭示了英国宏循环乳和甲耐药的cyathostomin群体的物种组成
K E Bull1, J Hodgkinson2, K Allen1
1Bristol Veterinary School, University of Bristol, Bristol, BS40 5DU, UK.
概括
草地虫的抗虫耐药性是特定于物种的,而不是多种物种的问题. 这项研究确定了与英国马匹耐药性相关的特定物种,影响了这些常见的马类寄生虫的治疗策略.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 马的健康 马的健康
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 赛亚托斯托明是常见的马类寄生虫,对虫剂的耐药性在全球范围内越来越令人担忧.
- 现有的治疗方法面临着已记录的耐药性,需要对潜在机制有更深入的了解.
- 之前的研究表明,英国纯血统的马匹具有耐药性,但特定物种的贡献尚不清楚.
研究的目的:
- 调查英国马匹对虫剂的物种特异性细胞质素耐药性.
- 为了确定与多药性耐药性和缩短卵子再出现周期相关的囊菌物种.
- 在治疗前和治疗后,量化对囊素群体进行分析.
主要方法:
- 在三年 (2021-2023) 期间,在英国四个马中收集来自马的强卵.
- 卵的共同培养到第三个幼虫阶段 (L3) 之前和之后的甲虫治疗.
- ITS-2区域的定量DNA元编码,以确定cyathostomin物种组成.
主要成果:
- 在一项研究中,特定的物种Cyathostomum catinatum和Cylicocyclus nassatus被确定为分别对宏环乳和甲耐药性负责.
- 卵子再出现时间缩短的子在治疗后的物种组成中显示出最小的变化.
- 这项研究提供了英国多药耐药性种群中第一种细胞质素物种的定量概况.
结论:
- 草地虫中虫耐药性是物种特异性的,挑战了多种现象的概念.
- 已识别的物种可能是耐药性的关键驱动因素,需要有针对性的控制策略.
- 这些发现对管理马类寄生虫耐药性和优化治疗方案有重大影响.
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