聚酸盐80和碳素甲基纤维素:在与微生物群相互作用时对上皮质完整性的不同影响
Alicia Bellanco1, Teresa Requena1, M Carmen Martínez-Cuesta1
1Department of Food Biotechnology and Microbiology, Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Nicolás Cabrera 9, 28049, Madrid, Spain.
概括
饮食乳化剂,如聚酸盐80 (P80) 和氧化甲基纤维素 (CMC) 改变肠道微生物组的组成和功能. 这些变化影响肠道上皮质完整性,需要在添加剂安全性评估中考虑微生物群.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 食品科学 食品科学 食品科学
背景情况:
- 饮食乳化剂,如聚酸盐80 (P80) 和氧化甲基纤维素 (CMC),是常见的食品添加剂.
- 有关它们的安全性和与肠道微生物群的潜在相互作用存在担忧.
研究的目的:
- 调查P80和CMC对肠道微生物群组成和功能的影响.
- 在肠道模拟器模型中评估这些乳化剂对肠道上皮质完整性的影响.
主要方法:
- 使用了一个动态的四阶段肠道微生物群模型 (BFBL肠道模拟器).
- 在微生物组分析中采用基于16S rDNA amplicon的元基因组学.
- 在暴露于乳化剂和微生物超剂后评估了上皮质完整性.
主要成果:
- 无论是P80还是CMC,都以剂量依赖的方式改变了微生物组合.
- 支持炎症的微生物群增加,而增强屏障的种群减少.
- P80降低了抗炎性种类 (Bacteroides dorei, Akkermansia);CMC增加了破坏屏障的种类 (Ruminococcus torques, Hungatella).
- 与其相关的微生物群和超级生物一起,CMC降低了上皮质完整性,与P80.0.不同.
结论:
- 食乳化剂P80和CMC显著影响肠道微生物群的结构和功能.
- 这些变化影响肠道上皮质完整性.
- 食品添加剂的风险评估应纳入肠道微生物群的作用.
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