Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptidoglycan Synthesis01:28

Peptidoglycan Synthesis

Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan...
Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers of polysaccharides that firmly attach to the bacterial cell wall. These structures serve as formidable protective barriers, preventing...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ecological vaccination: A strategy to prevent zoonotic spillover from bats.

Science advances·2026
Same author

Preclinical development of a well-defined semisynthetic glycoconjugate vaccine against <i>Haemophilus influenzae</i> type b.

Science advances·2026
Same author

Synergistic phototherapy and Ca<sup>2+</sup> consumption for combating biofilms in diabetic wounds <i>via</i> ion interference, physical disruption, and biological regulation.

Bioactive materials·2025
Same author

C-reactive protein is a broad-spectrum capsule-binding receptor for hepatic capture of blood-borne bacteria.

The EMBO journal·2025
Same author

Development and Biological Properties of a New Live Attenuated Mumps Vaccine Strain.

Vaccines·2025
Same author

Macroporous hydrogel loaded with AIE-photosensitizer for enhanced antibacterial and wounds healing.

International journal of biological macromolecules·2025

相关实验视频

Updated: Jun 3, 2025

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
09:11

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

Published on: September 25, 2014

21.8K

简化过程,用于制备Streptococcus pneumoniae的原生和脱聚合的囊多糖.

Yuelong Li1, Hantian Yao2, Yanli Liu1

  • 1Beijing Minhai Biotechnology Co. Ltd, Beijing 102600, China.

Carbohydrate polymers
|January 8, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种精简的工艺,用于净化Streptococcus pneumoniae囊多糖 (CPSs) 用于疫苗生产. 这种方法简化了杂质的去除和尺寸的缩小,确保高质量的CPS用于有效的肺炎球菌疫苗.

关键词:
囊中的多糖化物.不纯洁性 不纯洁性侵袭性肺炎球菌疾病的侵袭性肺炎球菌疾病核磁共振是一种核磁共振.聚糖的脱聚合法 聚糖的脱聚合法聚糖化物净化净化方法聚糖类疫苗 聚糖类疫苗肺炎链球菌的肺炎链球菌.

更多相关视频

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
13:53

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

4.9K
Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
04:25

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Published on: February 24, 2014

45.6K

相关实验视频

Last Updated: Jun 3, 2025

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
09:11

Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination

Published on: September 25, 2014

21.8K
Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
13:53

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

4.9K
Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
04:25

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Published on: February 24, 2014

45.6K

科学领域:

  • 微生物学 微生物学
  • 疫苗开发 疫苗开发
  • 生物化学 生物化学

背景情况:

  • 肺炎链球菌 (Streptococcus pneumoniae) 会导致严重的感染,如肺炎和脑膜炎.
  • 细菌囊多糖 (CPS) 是关键的毒性因素和疫苗的目标.
  • 目前的疫苗生产需要高度净化的原生和脱聚合的CPSs.

研究的目的:

  • 为了简化和优化Streptococcus pneumoniae囊多糖体 (CPSs) 的净化过程.
  • 为疫苗制造开发一种更有效的方法,以获得原生和脱聚合的CPS.
  • 为了确保CPS的纯度和结构完整性,必须符合严格的药库标准.

主要方法:

  • 实施了一种简化的净化协议,包括超过,一阶段酸沉和对本地CPS的透过.
  • 集成的三酸 (TFA) 水解用于有效地去聚合CPSs.
  • 根据传统方法和欧洲药典标准评估纯度和结构完整性.

主要成果:

  • 优化的过程成功地产生了纯化的原生和脱聚合的CPS.
  • 产生的CPS的纯度和结构完整性与通过传统的多步骤方法获得的可比.
  • 精简的流程简化了用于生产肺炎球菌疫苗的CPS净化.

结论:

  • 开发的方法为肺炎球菌疫苗的CPS净化提供了一种高效和有效的方法.
  • 这种优化的工艺符合疫苗制造所需的严格质量标准.
  • 精简的CPS净化对于持续和可扩展的肺炎球菌疫苗生产至关重要.