基于凝-氨基酸水凝的生物反应器,以优化酵母代谢物的生产
Nandita Srivastava1, Anirban Roy Choudhury1
1Biochemical Engineering Research & Process Development Centre (BERPDC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector-39A, Chandigarh 160036, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Carbohydrate polymers
|January 8, 2025
概括
这项研究引入了一种新的凝-氨基酸水凝用于固态发酵,其性能优于传统方法. 这种可持续的生物反应器有效地产生宝贵的代谢物,如β-胡卜素和脂质.
科学领域:
- 生物技术是生物技术.
- 生物材料科学 生物材料科学
- 微生物发酵 微生物发酵
背景情况:
- 水凝提供了模仿自然环境的水合,多孔矩阵,非常适合微生物生长和营养转移.
- 虽然用于细胞固定,但水凝尚未被探索为固态发酵的矩阵.
- 传统的水下发酵方法在效率和资源利用方面存在局限性.
研究的目的:
- 开发和评估一种使用凝和氨基酸进行固态发酵的创新型水凝生物反应器.
- 为了优化水凝成分,以提高Rhodosporidium sp.的代谢产量.
- 为了比较新型水凝系统的性能和经济性,与传统的浸泡发酵相比.
主要方法:
- 开发了一种凝-氨基酸水凝矩阵.
- 种植Rhodosporidium sp. 的方法 (MTCC 9733) 在生产代谢物的水凝中.
- 使用一次性一个因子和D-最佳响应表面方法优化凝和氨基酸度.
- 对β-胡卜素,脂质和蛋白质产量的评估.
- 在发酵前和后对水凝进行风湿学分析.
- 用浸泡发酵进行生产力,产量和工艺经济学的比较分析.
主要成果:
- 凝-氨基酸水凝系统成功支持了Rhodosporidium sp.的种植. 和代谢物生产.
- 优化的水凝条件导致β-胡卜素,脂质和蛋白质的显著产量.
- 新的固态水凝发酵与浸泡发酵相比,显示出更高的生产力,产量和工艺经济性.
- 优化的水凝具有出色的机械强度和结构完整性,适合多个发酵周期.
结论:
- 凝-氨基酸水凝是传统发酵方法的有希望,可持续和高效的替代品.
- 开发的水凝生物反应器在代谢物生产和工艺经济学方面具有优势.
- 该系统的稳定性支持其在工业发酵过程中重复使用的潜力.
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