基板尺度 一层一层的 Lactiplantibacillus plantarum WCFS1 的微封装
Nelson Romano1, Gina Tavares2, Stéphanie Passot3
1Center for Research and Development in Food Cryotechnology (CIDCA, CCT-CONICET), La Plata 1900, Argentina.
Food research international (Ottawa, Ont.)
|January 8, 2025
概括
优化乳酸细菌的层层封装提高了生存率. 这种可扩展的方法通过在加工和模拟肠道条件期间保护它们来增强细菌的传递.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 层层封装 (LbL) 保护乳酸细菌,但在实验室规模的效率和可扩展性方面面临挑战.
- 优化生物质生产和聚合物比率等参数对于成功扩展至关重要.
研究的目的:
- 为了优化LbL封装过程的乳植物菌植物 WCFS1在一个长椅生物反应器规模.
- 在封装和脱水后增强细菌的恢复和活力.
主要方法:
- 优化生物质生产 (葡萄糖补充,pH控制,早期静止阶段收获).
- 研究的聚合物处理 (奇托桑,酸盐) 和LbL参数 (生物质度,洗步骤,生物质与聚合物比率).
- 通过盘子计数评估封装效率,然后用载体 (FOS,马尔托德) 喷雾/冷干燥和模拟胃肠应力测试.
主要成果:
- 与不受控制的条件相比,受控生物质生产增加了超过3个日志单位的细菌恢复.
- 用最多两层聚合物 (B RainbowChi或B RainbowChi RainbowAlg) 涂层保持了可培养性;通过泽塔电位确定的最佳生物质与聚合物比率为10:1.
- LbL封装和载体化合物 (FOS,马尔托德素) 显示出对喷雾干燥和胃肠道压力的协同保护.
结论:
- 优化的LbL封装显著改善了乳酸细菌的恢复和生存能力.
- 开发的方法适用于扩大敏感细菌菌株的保护和传递.
- 这种方法有望提高益生菌在食品和治疗应用中的有效性.
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