甘拉丁协同地恢复了万科米辛的抗菌活性,对抗了对万科米辛耐药的Enterococcus faecium
Intu-Orn Ayamuang1, Yothin Teethaisong2, Kittipot Sirichaiwetchakoon3
1School of Biology, Institute of Science, Suranaree University of Technology, University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.
Journal of applied microbiology
|January 8, 2025
概括
天然化合物加拉 (GAL) 与万科米辛 (VAN) 结合,显示出对抗万科米辛耐药性菌 (Enterococcus faecium,VREfm) 的协同活性. 这种组合会破坏细菌细胞壁并抑制生物膜的形成,为VREfm感染提供了一种新的策略.
科学领域:
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
- 自然产品 化学 化学
背景情况:
- 菌 (Enterococcus faecium) 是一个重要的机会性病原体,具有越来越高的万科米辛耐药性 (VREfm).
- 组合疗法是克服抗生素耐药性的有希望的策略.
- 甘氨酸 (GAL) 是一种植物性生物活性化合物,具有潜在的抗微生物特性.
研究的目的:
- 评估加兰 (GAL) 和万科米 (VAN) 对抗抗万科米耐药的菌 (Enterococcus faecium,VREfm) 的协同活性.
- 研究GAL-VAN组合对VREfm的作用方式.
主要方法:
- 确定了VAN和GAL的最小抑制度 (MIC).
- 使用分数抑制度指数 (FICI) 评估了协同效应.
- 用于机械学研究的时间杀伤测试,电子显微镜和里埃变换红外光谱 (FTIR) 用于机械研究.
主要成果:
- VAN加GAL组合显示出对VREfm分离物的协同活性 (FICI:0.26-0.28).
- 机械学研究显示了膜透性受损,糖和膜损伤,以及生物膜形成的抑制.
- FTIR分析显示,生物膜中的脂质,蛋白质和碳水化合物含量减少.
结论:
- 甘拉丁 (GAL) 可以通过破坏细菌细胞外并抑制生物膜形成,恢复对VREfm的万科米辛 (VAN) 活性.
- GAL-VAN组合显示出作为治疗VREfm感染的辅助疗法的潜力.
- 进一步研究GAL作为现有抗生素的增强剂是有必要的.
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