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利用光激活的甲来对抗细胞内黄金葡萄球菌,使用体外角质细胞感染模型

Klaudia Szymczak¹, Michał Rychłowski², Lei Zhang³

¹Laboratory of Photobiology and Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdańsk, Poland.

Scientific reports

|January 8, 2025

概括

使用甲的抗微生物光动力失活剂 (aPDI) 有效地消除了细胞中的细胞内甲素耐药黄金葡萄球菌 (MRSA). 这种新的策略显示出治疗复发性感染的希望,而不会引起宿主细胞毒性.

科学领域:

微生物学微生物学
摄影化学的使用.
皮肤病学皮肤病学

背景情况:

黄金葡萄球菌 (S. aureus) 在角质细胞内细胞内持续存在,逃避抗生素并导致复发性感染.
需要有效的策略来根除细胞内,多药物耐药的细菌,如耐甲西林黄金色杆菌 (MRSA).

研究的目的:

评估抗微生物光动力学失活 (aPDI) 使用 (III) (Ga3+CHP) 和可见光对细胞内MRSA的疗效.
在感染模型中评估aPDI的实时毒性.

主要方法:

使用了一种感染MRSA的角质细胞模型.
(III) 氨酸 (Ga3+ CHP) 和可见光被用于aPDI.
使用流式细胞计和光显微镜分析了用Ga3+CHP对细菌的同位化,并评估了细菌的减少.

主要成果:

在受感染的角质细胞中积累的Ga3+CHP,在溶解体内与细胞内MRSA共定位.
aPDI治疗导致细胞内MRSA减少70%,并降低了细菌对宿主细胞的粘附.
细胞外MRSA水平也显著降低,aPDI显示没有显著的细胞毒性或光毒性.

结论:

用Ga3+CHP进行抗微生物光动力学失活 (aPDI) 是消除角质细胞中的细胞内MRSA的一个有希望的策略.
这种方法有效地减少了细胞内和细胞外细菌,而不会伤害宿主细胞.
aPDI为复发性黄金色杆菌感染提供了潜在的新疗法.

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